Abstract
It was shown that two stress factors, hypoxia and hyperosmotic shock, if applied simultaneously to the yeast Debaryomyces hansenii, display an antagonistic mode of interaction, which results in an increased degree of halophily of this microorganism under microaerobic conditions. Studies of the effects of respiration inhibitors (sodium azide and salicyl hydroxamic acid, SHA) and of the pattern of changes in the composition of the respiratory chain of Debaryomyces hansenii under the stated stress conditions led to the suggestion of three (or four) chains of electron transfer functioning simultaneously in the cell: the classical respiratory chain involving cytochrome-c oxidase, an alternative respiratory chain involving a cyanide-and azide-resistant oxidase, and additional respiratory chains involving oxidases resistant to salt, azide and SHA. Thus, the antagonistic mode of interaction between hypoxia and hyperosmotic shock results from the redirection of the electron flow from the salt-susceptible respiratory systems to the salt-unsusceptible ones encoded by “the hypoxia genes” and activated (induced) under microaerobic conditions.
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Original Russian Text © O. V. Shelemekh, O.V. Heidebrecht, V.K. Plakunov, S.S. Belyaev, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 4, pp. 562–569.
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Shelemekh, O.V., Heidebrecht, O.V., Plakunov, V.K. et al. “Oxygen regulation” of the respiratory chain composition in the yeast Debaryomyces hansenii under multiple stress. Microbiology 75, 486–493 (2006). https://doi.org/10.1134/S0026261706040205
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DOI: https://doi.org/10.1134/S0026261706040205