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Effect of the oligopeptide length on the DNA condensation process in aqueous-salt solutions

  • Proceedings of the 15th Symposium on Intermolecular Interactions and Conformations of Molecules Petrozavodsk, 2010, June 14–18
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Abstract

The methods of circular dichroism, ultraviolet spectrophotometry, dynamic light scattering, viscometry, dynamic birefringence, and atomic force microscopy are employed to study the conformational properties of the DNA molecule during its complexation with oligopeptides. Samples with c 2, 3, and 5 monomeric L-lysine residues are used. Experimental data evidence that in the diluted solutions (with a low ionic strength) of DNA with oligopeptides (with 3 and 5 monomeric units) the condensation of the macromolecule is observed at specific peptide concentrations. For the bipeptide the DNA condensation in the solution is not observed. In the DNA solutions with a high ionic strength (1 M NaCl), oligopeptides do not affect the macromolecule size.

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References

  1. V. Vijayanathan, T. Thomas, and T. J. Thomas, Biochem., 41, 14085–12094 (2002).

    Article  CAS  Google Scholar 

  2. A. V. Slita, N. A. Kasyanenko, O. V. Nazarova, et al., J. Biotechnol., 127, 679–693 (2007).

    Article  CAS  Google Scholar 

  3. R. P. Goodman, A. T. Schaap, and C. F. Tardin, Science, 310, 1661–1665 (2005).

    Article  CAS  Google Scholar 

  4. V. A. Bloomfield, Curr. Opin. Struct. Biol., 6, 334–341 (1996).

    Article  CAS  Google Scholar 

  5. M. J. McCauley, J. Zimmerman, et al., J. Mol. Biol., 374, 993–1004 (2007).

    Article  Google Scholar 

  6. N. Kasyanenko, N. Arikainen, and E. Frisman, Biophys. Chem., 70, 93–100 (1998).

    Article  CAS  Google Scholar 

  7. N. Kasyanenko, D. Afanasieva, B. Dribinsky, et al., Struct. Chem., 18, 519–525 (2007).

    Article  CAS  Google Scholar 

  8. L. C. Gosule and J. A. Schellman, Nature, 259, 333–335 (1976).

    Article  CAS  Google Scholar 

  9. J. Widom and R. L. Baldwin, J. Mol. Biol., 144, 431–443 (1980).

    Article  CAS  Google Scholar 

  10. H. J. Hansma, L. I. Pietrasanta, R. Golan, et al., J. Biomol. Struct. Dyn., 2, 271–275 (2000).

    Google Scholar 

  11. I. Nayvelt, T. Thomas, and T. J. Thomas, Biomacromol., 8, 477–484 (2007).

    Article  CAS  Google Scholar 

  12. É. V. Frisman, L. V. Shchagina, and V. I. Vorob’ev, Kolloid. Zh., 27, 130–134 (1965).

    CAS  Google Scholar 

  13. É. V. Frisman, M. A. Sibileva, and A. V. Krasnoperova, Vysokomol. Soedin., 1, 597–606 (1959).

    CAS  Google Scholar 

  14. N. A. Kasyanenko, N. E. D’yakonova, and É. V. Frisman, Mol. Biol., 23, No. 4, 835–841 (1989).

    Google Scholar 

  15. N. A. Kasyanenko and B. A. Dribinskii, J. Struct. Chem., 48, No. 4, 729–733 (2007).

    Article  CAS  Google Scholar 

  16. N. A. Kasyanenko, D. A. Mukhin, and I. L. Perevyazko, Vysokomol. Soedin., Ser. A, 52, No. 7, 1–13 (2010).

    Google Scholar 

  17. N. A. Kasyanenko, A. V. Zanina, O. V. Nazarova, et al., Langmuir., 15, 7912–7917 (1999).

    Article  CAS  Google Scholar 

  18. V. Vijayanathan, T. Thomas, A. Shirakhata, et al., Biochem., 40, 13644–13651 (2001).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Physics Department, St. Petersburg State University, St. Petersburg, Russia

    B. A. Dribinskii & N. A. Kasyanenko

Authors
  1. B. A. Dribinskii
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  2. N. A. Kasyanenko
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Correspondence to B. A. Dribinskii.

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Original Russian Text Copyright © 2011 by B. A. Dribinskii and N. A. Kasyanenko

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Translated from Zhurnal Strukturnoi Khimii, Vol. 52, No. 6, pp. 1239–1245, November–December, 2011.

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Dribinskii, B.A., Kasyanenko, N.A. Effect of the oligopeptide length on the DNA condensation process in aqueous-salt solutions. J Struct Chem 52, 1202–1208 (2011). https://doi.org/10.1134/S002247661106028X

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  • DOI: https://doi.org/10.1134/S002247661106028X

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