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Extracellular Matrix Alterations in Human Soleus Muscle under Conditions of Chronic Disorder of Consciousness

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Abstract

Critical illness myopathy (CIM) is a primary myopathy that develops in critically ill patients. Histological features of CIM include a general reduction in muscle fiber cross-sectional area and a predominant loss of the motor protein myosin in the absence of inflammatory infiltrates but with detectable cytokine activation. This study was aimed to examine the state of the soleus muscle extracellular matrix in patients with CIM caused by chronic disorders of consciousness. Incisional needle biopsies of the soleus muscle were taken from 6 patients with a chronic (≥ 2 months) disorder of consciousness, undergoing treatment at the Polenov Neurosurgical Institute (Almazov National Medical Research Center, St. Petersburg), and healthy men (control). Histological staining of soleus muscle sections in patients with CIM revealed a significantly increased collagen area that exceeded control values by 82%. Type I, III and VIa collagen mRNA levels, along with type I and III collagen content, were increased. No changes were found in fibronectin and extracellular tissue growth factor mRNA levels. However, integrin α7 mRNA levels were elevated. Our results indicate significant skeletal muscle fibrosis in CIM, requiring further studies on the signaling pathways that regulate this process.

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ACKNOWLEDGMENTS

The authors are deeply grateful to E.M. Lednev and T.F. Vepkhvadze for conducting needle biopsy, as well as K.K. Kukanov and N.E. Voinov for biopsy sampling.

Funding

This work was supported by the Russian Science Foundation (project No. 22-25-00615). No other grants were obtained to carry out or supervise this particular research.

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    K. A. Zaripova, S. A. Tyganov, O. V. Turtikova, E. N. Skiteva & B. S. Shenkman

  2. Department of Anesthesiology and Intensive Care, Russian National Institute of Chemistry, St. Petersburg, Russia

    E. N. Skiteva, Y. M. Zabrodskaya, E. A. Kondratieva & A. N. Kondratiev

  3. Mechnikov North-Western State Medical University, St. Petersburg, Russia

    Y. M. Zabrodskaya

  4. Kirov Military Medical Academy, St. Petersburg, Russia

    Y. M. Zabrodskaya & E. A. Kondratieva

  5. AIMIS Healthcare Group, Сlinic Athonitissa, Limassol, Cyprus

    S. A. Kondratiev

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  1. K. A. Zaripova
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  2. S. A. Tyganov
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  3. O. V. Turtikova
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Contributions

Conceptualization and experimental design (B.S.Sh., A.N.K., Yu.M.Z., S.A.K.), muscle biopsy (S.A.K., E.N.S.), data collection (S.A.T., E.N.S., K.A.Z., O.N.T.), data processing (S.A.T., K.A.Z., O.N.T.), writing and editing the manuscript (S.A.T., B.S.Sh., E.A.K., S.A.K., Yu.M.Z.).

Corresponding author

Correspondence to S. A. Tyganov.

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ETHICS APPROVAL

All studies were conducted in accordance with the principles of biomedical ethics set forth in the 1964 Declaration of Helsinki and its subsequent amendments. They were also approved by the local Ethics Committee of the Almazov National Medical Research Center, Minutes No. 1411-20 of November 16, 2020. Muscle biopsy sampling was performed for diagnostic purposes with the written consent of the patients’ legal representatives.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

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Translated by A. Polyanovsky

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Zaripova, K.A., Tyganov, S.A., Turtikova, O.V. et al. Extracellular Matrix Alterations in Human Soleus Muscle under Conditions of Chronic Disorder of Consciousness. J Evol Biochem Phys 60, 432–442 (2024). https://doi.org/10.1134/S0022093024010332

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