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Reconstruction of Metabolic Activity of the Gut Microbiota in Children and Adults with Obesity and Its Relationship with the Representation of Alkylresorcinols in Stool Samples

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Abstract

The human body is a superorganism whose performance depends on trillions of residential microbial cells. Evolving together with the host, the microbiota formed the phenotypes of our ancestors. Mutations emerging in the process of natural selection led to a co-adaptation of the host organism and microbial cells to environmental conditions. The composition and metabolic activity of the gut microbiota influence multiple physiological processes, as well as the development of pathological conditions and dysmetabolic syndrome that includes obesity. Alkylresorcinols (ARs) are biologically active polyphenolic compounds, mainly plant- or microbial-derived, that have the ability to highly affect host metabolism and the composition of the gut microbiota. In the present study, we carried out metagenomic sequencing of microbial DNA isolated from the stool samples of 401 metabolically healthy and obese children and adults, and also determined the content of various AR homologs in these samples. Using the high-throughput sequencing data, we reconstructed the metabolic potential of the gut microbiota and assessed the correlations between the content of various AR homologs and the representation of bacterial genes encoding various enzymes (KEGG database) in stool samples. Based on the results obtained, we revealed the features of functional changes in the intestinal microbiota in adults and children with obesity.

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Funding

This research was state budget funded.

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Authors and Affiliations

  1. Pirogov Russian National Research Medical University, Academic Chair of Biochemistry and Molecular Biology, Faculty of General Medicine, Moscow, Russia

    A. A. Zabolotneva, A. B. Itov & A. V. Shestopalov

  2. Medical Research Center of Endocrinology, Moscow, Russia

    A. A. Zabolotneva, S. A. Roumiantsev & A. V. Shestopalov

  3. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

    A. B. Itov & A. V. Shestopalov

  4. Kazan Federal University, Kazan, Russia

    T. V. Grigorieva & I. Yu. Vasiliev

  5. Centre for Digital and Translational Biomedicine LLC “Centre for molecular health”, Moscow, Russia

    S. A. Roumiantsev & A. M. Gaponov

Authors
  1. A. A. Zabolotneva
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  2. A. B. Itov
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  3. T. V. Grigorieva
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  4. I. Yu. Vasiliev
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  5. S. A. Roumiantsev
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  6. A. M. Gaponov
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  7. A. V. Shestopalov
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Contributions

Conceptualization and experimental design (A.V.Sh., T.V.G.), data collection (I.Yu.V., A.M.G.), data processing (A.A.Z., A.B.I.), writing and editing a manuscript (A.A.Z., S.A.R., A.V.Sh.).

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Correspondence to A. A. Zabolotneva.

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The authors declare that they have no conflict of interest related to the publication of this article.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2023, Vol. 59, No. 4, pp. 271–291https://doi.org/10.31857/S0044452923040095.

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Zabolotneva, A.A., Itov, A.B., Grigorieva, T.V. et al. Reconstruction of Metabolic Activity of the Gut Microbiota in Children and Adults with Obesity and Its Relationship with the Representation of Alkylresorcinols in Stool Samples. J Evol Biochem Phys 59, 1037–1056 (2023). https://doi.org/10.1134/S002209302304004X

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