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Peculiarities of Ca2+-regulation of functional activity of myocardium of frog Rana temporaria

  • Comparative and Ontogenic Physiology
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Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

To elucidate role of intra- and extracellular Ca2+ in regulation of rhythm and strength of frog heart contractions, there were studied ECC and isometric contraction of myocardium preparations in response to verapamil, adrenaline, and blockers of α- and β-adrenoreceptors. It has been shown that after an intramuscular injection of verapamil (6 mg/kg), bradycardia develops, the heart rate (HR) decreasing by 50–70%. Further, the cardiac arrest occurred; however, administration to the animals of adrenaline (100 mg/kg) restored the cardiac rhythm for a short while. After an intramuscular injection of adrenaline at doses of 0.1–10 mg/kg, no essential changes were observed in the potential action amplitude and HR; an increase of the administered adrenalin concentration to 100 mg/kg was not accompanied by the cardiac rhythm stimulation, as this takes place in homoiothermal animals and human; on the contrary, an essential HR deceleration was revealed. Phentolamine (5 mg/kg) gradually decelerated HR rhythm by 32–45%. The potential amplitude changed insignificantly. A subsequent intracardiac injection of adrenaline (100 mg/kg) on the background of block of α-adrenoreceptors produced acceleration of the rhythm (by 15–21%) and fall of the electrogram amplitude. These results can indicate that in the frog heart phentolamine interacts predominantly with α 1-adrenoreceptors. An intracardial administration of propranolol (1 mg/kg) to frogs promoted inhibition of β-adrenergic receptors and produced a gradual cardiac rhythm deceleration. In experiments on assessment of verapamil effect on the character of contractions this preparation at a concentration of 150 μM was established to produce a significant dose-dependent decrease of the contraction strength. A rise of verapamil concentration in the sample to 200 μM led to a decrease of the amplitude, on average, by 68–70% and in individual preparations—by 80–85%; however, administration into the sample of adrenaline (10 μM) restored the cardiac contraction strength. Adrenaline (1 nM–100 μM) increased markedly the contraction amplitude. Phentolamine (10 μM) did not inhibit transmission of contractile signal to cardiomyocytes; this was manifested in that the contraction amplitude after addition of adrenaline (10 μM) into the sample was approximately the same as in the sample containing no phentolamine. Propranolol (10 μM) eliminated the stimulatory action of adrenaline (10 μM). The results of these experiments indicate that in the frog ventricular cardiomyocytes the main adrenaline acceptors are β-adrenoreceptors.

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    I. V. Shemarova, S. V. Kuznetsov, I. N. Demina & V. P. Nesterov

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  1. I. V. Shemarova
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  2. S. V. Kuznetsov
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  3. I. N. Demina
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Original Russian Text © I. V. Shemarova, S. V. Kuznetsov, I. N. Demina, V. P. Nesterov, 2008, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2008, Vol. 44, No. 1, pp. 39–50.

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Shemarova, I.V., Kuznetsov, S.V., Demina, I.N. et al. Peculiarities of Ca2+-regulation of functional activity of myocardium of frog Rana temporaria . J Evol Biochem Phys 44, 44–57 (2008). https://doi.org/10.1134/S0022093008010064

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  • DOI: https://doi.org/10.1134/S0022093008010064

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