Abstract
A calcium cell signaling system is one of the first, which were formed in the course of evolution of systems. The understanding of calcium binding–uncaging dynamics is crucial in studies of corresponding intracellular processes. By now, a great number of calcium-dependent processes have been investigated. However, works that fully consider these processes are absent. This is specified in many respects by the instrumental abilities. In this work, requirements for the experimental setup intended for comprehensive studies of calcium interaction dynamics are briefly formulated, its block diagram is described, and the results of test experiments are presented.
Similar content being viewed by others
References
Faas, G.C., Raghavachari, S., Lisman, J.E., and Mody, I., Nat. Neurosci., 2011, vol. 14, no. 3, pp. 301–304. doi 10.1038/nn.2746
Murakoshi, H. and Yasuda, R., Trends Neurosci., 2012, vol. 35, no. 2, pp. 135–143. doi 10.1016/jtins.2011. 12.002
Escobar, A.L., Velez, P., Kim, A.M., Cifuentes, F., Fill, M., and Vergara, J.L., Pflügers Arch.–Eur. J. Physiol., 1997, vol. 434, no. 5, pp. 615–631. doi 10.1007 /s004240050444
Bensasson, R.V., Land, E.J., and Truskot, T.G., Flash Photolysis and Pulse Radiolysis. Contribution to the Chemistry of Biology and Medicine, Pergamon, 1983.
Stutzmann, G.E., LaFerla, F.M., and Parker, I., J. Neurosci., 2003, vol. 23, no. 3, pp. 758–765.
Nägerl, U.V., Novo, D., Mody, I., and Vergara, J.L., Biophys. J., 2000, vol. 79, no. 6, pp. 3009–3018. doi 10.1016/S0006-3495(00)76537-4
Blehm, B.H. and Selvin, P.R., Chem. Rev., 2014, vol. 114, no. 6, pp. 3335–3352. doi 10.1021/cr4005555
Sitnikov, D.S., Ovchinnikov, A.V., Ilina, I.V., Chefonov, O.V., and Agranat, M.B., High Temp., 2014, vol. 52, no. 6, pp. 803–808. doi 10.1134/ s0018151x14060200
Neuman, K.C. and Block, S.M., Rev. Sci. Instrum., 2004, vol. 75, no. 9, pp. 2787–2809. doi 10.1063/ 1.1785844
Brouhard, G.J., Schek, H.T., and Hunt, A.J., IEEE Trans. Biomed. Eng., 2003, vol. 50, no. 1, pp. 121–125. doi 10.1109/tbme.2002.805463
Perkins, T.T., Dalal, R.V., Mitsis, P.G., and Block, S.M., Science, 2003, vol. 301, no. 5641, pp. 1914–1918. doi 10.1126/science.1088047
Koch, S.J., Shundrovsky, A., Jantzen, B.C., and Wang, M.D., Biophys. J., 2002, vol. 83, no. 2, pp. 1098–1105. doi 10.1016/s0006-3495(02)75233-8
Mehta, A.D., Rief, M., Spudich, J.A., Smith, D.A., and Simmons, R.M., Science, 1999, vol. 283, no. 5408, pp. 1689–1695.
Svoboda, K. and Block, S.M., Ann. Rev. Biophys. Biomol. Struct., 1994, vol. 23, no. 1, pp. 247–285. doi 10.1146/annurevbb.23.060194.001335
Mehta, A.D., Finer, J.T., and Spudich, J.A., Methods Enzymol., 1998, vol. 298, pp. 436–459.
Simmons, R.M., Finer, J.T., Chu, S., and Spudich, J.A., Biophys. J., 1996, vol. 70, no. 4, pp. 1813–1822. doi 10.1016/s0006-3495(96)79746-1
Lukinavicius, G., Reymond, L., D’Este, E., Masharina, A., Gottfert, F., Ta, H., Guther, A., Fournier, M., Rizzo, S., Waldmann, H., Blaukopf, C., Sommer, C., Gerlich, D.W., Arndt, H.-D., Hell, S.W., et al., Nat. Meth., 2014, vol. 11, no. 7, pp. 731–733. doi 10.1038/nmeth.2972
Iwaki, M., Iwane, A.H., Ikezaki, K., and Yanagida, T., Nano Lett., 2015, vol. 15, no. 4, pp. 2456–2461. doi 10.1021/nl5049059
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.Yu. Loktionov, M.G. Mikhaylova, D.S. Sitnikov, 2016, published in Pribory i Tekhnika Eksperimenta, 2016, No. 4, pp. 124–129.
Rights and permissions
About this article
Cite this article
Loktionov, E.Y., Mikhaylova, M.G. & Sitnikov, D.S. Experimental setup for studying dynamics of the calcium interaction in cells. Instrum Exp Tech 59, 601–606 (2016). https://doi.org/10.1134/S0020441216030076
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0020441216030076