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Specific and Nonspecific Interactions of Yersinia pseudotuberculosis Lipopolysaccharide with Monoclonal Antibodies Assessed by Atomic Force Microscopy

  • MOLECULAR BIOPHYSICS
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Abstract

Atomic force microscopy was used to evaluate the rupture force between a probe functionalized with the lipopolysaccharide from Yersinia pseudotuberculosis and monoclonal antibodies immobilized on mica as well as the contribution of non-specific factors to such interaction. There were no strongly indicated interactions between the “clean” probe and the treated or “clean” mica. When the last one was modified with (3-aminopropyl)triethoxysilane (APTES), the interaction force increased becoming even greater if the aminated mica was treated with glutaraldehyde. Subsequent immobilization of monoclonal antibodies on the APTES and glutaraldehyde-coated mica weakened the interaction dramatically, which was much less pronounced when complementary monoclonal antibodies used in comparison with heterologous ones. The specific interaction between antibodies and aggregation-prone antigens (e.g. lipopolysaccharides) may not exceed the nonspecific one mediated by “disaggregation,” ”unfolding” of the surface-immobilized molecular agglomerates on the probe and mica.

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Funding

This research was carried out with the financial support of the Russian Foundation for Basic Research, project no. 20-34-90013, as well as a grant from the president of the Russian Federation for State Support of Young Russian Scientists-Candidates of Sciences, project no. MK-3383.2021.1.4.

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Authors and Affiliations

  1. Vyatka State University, 610000, Kirov, Russia

    A. A. Byvalov, V. S. Belozerov, B. A. Ananchenko & I. V. Konyshev

  2. Institute of Physiology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982, Syktyvkar, Russia

    A. A. Byvalov, V. S. Belozerov & I. V. Konyshev

Authors
  1. A. A. Byvalov
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  2. V. S. Belozerov
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  3. B. A. Ananchenko
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  4. I. V. Konyshev
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Correspondence to A. A. Byvalov.

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Statement of the welfare of humans or animals. The article does not contain any studies involving humans or animals in experiments performed by any of the authors..

Additional information

Translated by E. Puchkov

Abbreviations: AFM, atomic force microscopy; LPS, lipopolysaccharide; mAb, monoclonal antibodies; APTES, (3-aminopropyl)triethoxysilane; GA, glutaraldehyde; PBS, phosphate buffer solution.

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Byvalov, A.A., Belozerov, V.S., Ananchenko, B.A. et al. Specific and Nonspecific Interactions of Yersinia pseudotuberculosis Lipopolysaccharide with Monoclonal Antibodies Assessed by Atomic Force Microscopy. BIOPHYSICS 67, 856–866 (2022). https://doi.org/10.1134/S0006350922060033

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  • DOI: https://doi.org/10.1134/S0006350922060033

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