Abstract
Atomic force microscopy (AFM) is being increasingly used to directly measure protein interactions in nearly physiological environments. Here, protocols for atomic force microscopy (AFM) for visualization of antigen–antibody complexes are presented. The technique is used to demonstrate complexes formed by rheumatoid arthritis patient antibodies with lipopolysaccharide (LPS) isolated from P. mirabilis (O3) strain S1959 and a synthetic antigen (LPS epitope of 6 N-alpha-(D-galacturonoyl)-L-lysine residues).
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Acknowledgments
The presented studies were supported by grant “Preludium 8, UMO-2014/15/N/NZ6/02505,” National Research Center, Poland for JG-O, and BS 2018 UJK for WK.
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Kaca, W., Gleńska-Olender, J., Konieczna, I., Gawęda, J., Sęk, S. (2019). Assessment of Proteus mirabilis Antigen Immunological Complexes by Atomic Force Microscopy. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_23
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DOI: https://doi.org/10.1007/978-1-4939-9601-8_23
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