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Hydration effects accompanying the formation of DNA complexes with some ligands

  • Molecular Biophysics
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Abstract

In the range of millimeter wavelengths the dielectric properties of aqueous solutions of some biologically active ligands (potential anticarcinogen chlorophyllin; pharmacological drug caffeine; polyamine putrescine; mutagens proflavine and ethidium bromide; actinocin derivative, an analogue of antitumor antibiotic actinomycin D) and DNA complexes with these substances were studied. It was shown that complex formation is accompanied by the change in dielectric properties of the solution. These changes during interaction of DNA with the first three compounds correspond to a decrease in hydration (compared with the total hydration of free components), and in other cases they cause an increase in hydration. The number of water molecules bound with both the ligand and DNA nucleotide in the complex was estimated. The results were compared with existing models of DNA interaction with the studied substances.

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Authors and Affiliations

  1. Usikov Institute for Radiophysics and Electronics, National Academy of Sciences of Ukraine, Kharkov, 61085, Ukraine

    V. A. Kashpur, O. V. Khorunzhaya, D. A. Pesina, A. V. Shestopalova & V. Ya. Maleev

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  1. V. A. Kashpur
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  2. O. V. Khorunzhaya
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  3. D. A. Pesina
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  4. A. V. Shestopalova
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  5. V. Ya. Maleev
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Correspondence to D. A. Pesina.

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Original Russian Text © V.A. Kashpur, O.V. Khorunzhaya, D.A. Pesina, A.V. Shestopalova, V.Ya. Maleev, 2017, published in Biofizika, 2017, Vol. 62, No. 1, pp. 39–46.

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Kashpur, V.A., Khorunzhaya, O.V., Pesina, D.A. et al. Hydration effects accompanying the formation of DNA complexes with some ligands. BIOPHYSICS 62, 31–37 (2017). https://doi.org/10.1134/S0006350917010092

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  • DOI: https://doi.org/10.1134/S0006350917010092

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