Skip to main content
SpringerLink
Log in

The transport activity of P-glycoprotein upon a change of the redox balance in lymphocytes of patients with chronic B-lymphocytic leukemia

  • Cell Biophysics
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract

The effect of drugs used in the treatment of chronic B-lymphocytic leukemia on the functional activity of P-glycoprotein, which is a membrane transporter associated with the phenomenon of multiple drug resistance, in B-lymphocytes of leukemia patients has been investigated. The level of reactive oxygen species and the content of low molecular weight antioxidants in the leukemic cells during the metabolic processing of chemotherapy drugs has been assessed. The degree of involvement of low molecular weight antioxidants in the maintenance of redox balance in the lymphocytes of drug-treated leukemia patients has been characterized. Variation of the levels of reactive oxygen species in leukemic B-lymphocytes within a certain range, as well as the subsequent return of these values to the levels of intact cells, was shown to enhance the P-glycoprotein transport activity. The viability of leukemic B-cells decreased upon exposure to antitumor compounds and depended on the redox balance. No statistically significant correlation between the P-glycoprotein transport activity and the viability of lymphocytes during the detoxification from drugs in B-cell chronic lymphocytic leukemia patients has been detected; however, the transport activity of the protein was directly correlated to the viability of leukemia cells within 15 h after exposure of the cells to H2O2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

RB:

redox balance

MDR:

multidrug resistance

ROS:

reactive oxygen species

B-CLL:

chronic B-lymphocytic leukemia

P-gp:

P-glycoprotein

LMWA:

low molecular weight antioxidants

NAC:

N-acetyl-L-cysteine

References

  1. M. Wartenberg, E. Hoffmann, H. Schwindt, et al., FEBS Lett. 579 (20), 4541 (2005).

    Article  Google Scholar 

  2. N. V. Ermakova, Candidate’s Dissertation in Biology (Pushchino, 2005).

    Google Scholar 

  3. M. T. Kuo, Antioxid. Redox. Signal. 11 (1), 99 (2009).

    Article  Google Scholar 

  4. W.-S. Jin, Zh. Kong, Zh. Shen, et al., J. Exp. Clin. Cancer Res. 30 (61), 1 (2011). http://www.jeccr.com/ content/30/1/61/-ins2.

    Google Scholar 

  5. M. O. Emelyanov, Yu. A. Kim, A. F. Korystova, and L. N. Kublik, Biol. Membrany 27 (3), 244 (2010).

    Google Scholar 

  6. A. V. Tamashevski, Y. M. Harmaza, E. I. Slobozhanina, and A. I. Svirnovskii, Novosti Med. Biol. Nauk 6 (4), 173 (2012).

    Google Scholar 

  7. N. Gadol, G. Nakamura, and A. Saunders, Diagn. Immunol. 3 (3), 145 (1985).

    Google Scholar 

  8. J. Chandra, J. Hackbarth, S. Le, et al., Blood 102 (13), 4512 (2003).

    Article  Google Scholar 

  9. Sigma–Aldrich Antioxidant Assay Kit, http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/cs0790bul.pdf.

  10. P. F.R. Palma, G. L. Baggio, C. Spada, et al., Brazil. J. Infectious Diseases 12 (2), 108 (2008).

    Article  Google Scholar 

  11. E. B. Mechetner, B. Schott, B. S. Morse, et al., Biochemistry 94 (24), 12908 (1997).

    Google Scholar 

  12. E. Cabezudo, E. Matutes, M. Ramrattan, et al., Leukemia 11 (11), 909 (1997).

    Google Scholar 

  13. D. D. Wayner, G. W. Burton, K. U. Ingold, and S. Locke, FEBS Lett. 187 (1), 33 (1985).

    Article  Google Scholar 

  14. R. Silber, C. M. Farber, E. Papadopoulos, et al., Blood 80 (8), 2038 (1992).

    Google Scholar 

  15. G. Szakács, J. K. Paterson, J. A. Ludwig, et al., Nature Rev. Drug Discov. 5 (3), 219 (2006).

    Article  Google Scholar 

  16. C. A. Koczor, R. A. Torres, and W. Lewis, Expert Opin. Drug Metab. Toxicol. 8 (6), 665 (2012).

    Article  Google Scholar 

  17. D. Fu, M. Bebawy, E. P. W. Kable, et al., Int. J. Cancer 109 (2), 174 (2004).

    Article  Google Scholar 

  18. Y. Yang, Y. Liu, Z. Dong, et al., J. Biol. Chem. 282 (12), 8821 (2007).

    Article  Google Scholar 

  19. A. A. Stavrovskaya, Biol. Membrany 20 (3), 196 (2003).

    Google Scholar 

  20. M. J. Smit and R. Anderson, Agents Actions 36 (1–2), 58 (1992).

    Article  Google Scholar 

  21. R. Jitschin, A. D. Hofmann, H. Bruns, et al., Blood 123 (17), 2663 (2014).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, ul. Akademicheskaya 27, Minsk, 220072, Belarus

    A. V. Tamashevski, Y. M. Harmaza & E. I. Slobozhanina

  2. Republican Research and Therapy Center of Transfusion and Medical Biotechnology, Dolginovskii trakt 160, Minsk, 220053, Belarus

    A. I. Svirnovski

Authors
  1. A. V. Tamashevski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. M. Harmaza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. I. Slobozhanina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. I. Svirnovski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Tamashevski.

Additional information

Original Russian Text © A.V. Tamashevski, Y.M. Harmaza, E.I. Slobozhanina, A.I. Svirnovski, 2016, published in Biofizika, 2016, Vol. 61, No. 6, pp. 1173–1181.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tamashevski, A.V., Harmaza, Y.M., Slobozhanina, E.I. et al. The transport activity of P-glycoprotein upon a change of the redox balance in lymphocytes of patients with chronic B-lymphocytic leukemia. BIOPHYSICS 61, 971–978 (2016). https://doi.org/10.1134/S0006350916060257

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350916060257

Keywords

Search

Navigation