Abstract
Lymphocytes in blood smears of children diagnosed with cancer have been investigated and compared to those of healthy children (matching controls). The conventional hematological procedure for smear staining was used to assess the blood formula and lymphocyte indices, and a new staining method involving incubation of cells in a biochemical medium for 1 h was used to assess the activity of succinate dehydrogenase, lactate dehydrogenase, and the ratio of the activities of these enzymes characterizing the interaction of respiration and glycolysis. A dramatic increase of the size of lymphocytes obtained from cancer patients was detected when lactic acid was added to the incubation medium for the biochemical reaction. This effect was neither observed when succinic acid was added nor when blood smears from healthy children of the control group were tested. Structural changes in the patients’ cells were associated with changes in the activity of the enzymes under investigation. The cytobiochemical test that was developed allows increasing diagnostic sensitivity upon assessment of the patients’ state and can contribute to more efficient treatment of malignant tumors.
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Abbreviations
- SDH:
-
succinate dehydrogenase
- LDH:
-
lactate dehydrogenase
- LII:
-
leukocyte intoxication indices (described in detail in the text)
- LSI:
-
leukocyte shear index
- NLRI:
-
neutrophil-lymphocyte ratio index
- LMRI:
-
lymphocyte-monocyte ratio index
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Original Russian Text © N.V. Khunderyakova, S.A. Plyasunova, E.G. Litvinova, T.V. Yatchkula, M.V. Zakharchenko, A.V. Kovsan, N.I. Fedotcheva, P.M. Schwartsburd, M.N. Kondrashova, 2014, published in Biofizika, 2014, Vol. 59, No. 6, pp. 1101–1107.
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Khunderyakova, N.V., Plyasunova, S.A., Litvinova, E.G. et al. Changes in lymphocytes induced by oxidation substrates. BIOPHYSICS 59, 894–899 (2014). https://doi.org/10.1134/S0006350914060050
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DOI: https://doi.org/10.1134/S0006350914060050