Abstract
The effect of spermine on Ca2+-dependent permeability transition in mitochondria and liposomes induced by palmitic and α,ω-hexadecanedioic acid was studied. It has been shown that spermine inhibited the cyclosporin A-insensitive mitochondrial swelling induced by palmitic acid and Ca2+ and α,ω-hexadecanedioic acid and Ca2+. 100 μM spermine did not influence the mitochondrial respiration in state V2 and the respiration stimulated by palmitic acid, α,ω-hexadecanedioic acid and Ca2+. Preincubation of liposomes with 100 μM spermine resulted in inhibition of the palmitic acid/Ca2+- and α,ω-hexadecanedioic acid/Ca2+-induced release of a fluorescent dye sulforhodamine B from liposomes. At the same time, spermine added to fatty acid-containing liposomes stimulated Ca2+-dependent release of sulforhodamine B from liposomes. Addition of spermine to liposomes resulted in a significant increase in the ζ -potential of liposomal membranes (from −39.8 to −18.6 mV). A possible mechanism of spermine influence on palmitic acid/Ca2+- and α,ω-hexadecanedioic acid/Ca2+-induced permeability transition in mitochondria and liposomes is discussed.
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Original Russian Text © K.N. Belosludtsev, N.V. Belosludtseva, M.V. Dubinin, S.V. Gudkov, N.V. Penkov, V.N. Samartsev, 2014, published in Biofizika, 2014, Vol. 59, No. 5, pp. 895–901.
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Belosludtsev, K.N., Belosludtseva, N.V., Dubinin, M.V. et al. The influence of spermine on Ca2+-dependent permeability transition in mitochondria and liposomes induced by palmitic and α,ω-hexadecanedioic acids. BIOPHYSICS 59, 727–731 (2014). https://doi.org/10.1134/S0006350914050042
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DOI: https://doi.org/10.1134/S0006350914050042