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Electrochemical measurement of intraprotein and interprotein electron transfer

  • Molecular Biophysics
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Abstract

Intramolecular and intermolecular direct (unmediated) electron transfer was studied by electrochemical techniques in a flavohemoprotein cytochrome P450 BM3 (CYP102A1 from Bacillius megaterium) and between cytochromes b 5 and c. P450 BM3 was immobilized on a screen printed graphite electrode modified with a biocompatible nanocomposite material based on didodecyldimethylammonium bromide (DDAB) and gold nanoparticles. Analytical characteristics of SPG/DDAB/Au/P450 BM3 electrodes were studied with cyclic voltammetry and square wave voltammetry. The electron transport chain in P450 BM3 immobilized on the nanostructured electrode is: electrode → FAD → FMN → heme; i.e., electron transfer takes place inside the cytochrome, in evidence of functional interaction between its diflavin and heme domains. The effects of substrate (lauric acid) or inhibitor (metyrapone or imidazole) binding on the electro-chemical parameters of P450 BM3 were assessed. Electrochemical analysis has also demonstrated intermolecular electron transfer between electrode-immobilized and soluble cytochromes properly differing in redox potentials.

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russia

    V. V. Shumyantseva, T. V. Bulko, A. V. Kuzikov, E. V. Suprun & A. I. Archakov

  2. Russian National Medical University, Moscow, 117997, Russia

    V. B. Lisitsyna

  3. Henrich-Heine-University of Duesseldorf, Duesseldorf, North Rhine-Westfalia, 40225, Germany

    V. B. Urlacher

Authors
  1. V. V. Shumyantseva
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  2. T. V. Bulko
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  3. V. B. Lisitsyna
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  4. V. B. Urlacher
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  5. A. V. Kuzikov
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  6. E. V. Suprun
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  7. A. I. Archakov
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Correspondence to V. V. Shumyantseva.

Additional information

Original Russian Text © V.V. Shumyantseva, T.V. Bulko, V.B. Lisitsyna, V.B. Urlacher, A.V. Kuzikov, E.V. Suprun, A.I. Archakov, 2013, published in Biofizika, 2013, Vol. 58, No. 3, pp. 453–460.

The text and presentation had to be additionally revised for the English version. A.G.

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Shumyantseva, V.V., Bulko, T.V., Lisitsyna, V.B. et al. Electrochemical measurement of intraprotein and interprotein electron transfer. BIOPHYSICS 58, 349–354 (2013). https://doi.org/10.1134/S0006350913030172

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  • DOI: https://doi.org/10.1134/S0006350913030172

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