Abstract
This chapter addresses bioelectrochemical processes associated with the electrode/enzyme interface, examining an enzyme’s ability to exchange electrons directly with an electrode surface. Knowledge of the physical and chemical properties of a redox protein is fundamental for an effective, fast, direct exchange of electrons. Essential parameters in the immobilization method include the side through which the enzyme adsorbs on the electrode as well as the distance between the active site and the electrode surface. Theoretical parameters foreseen in Marcus theory are also key. Still, some enzymes cannot perform direct electron transfer—in this case, mediating species are used. This process is known as mediated electron transfer. Such species act by bringing the site around the enzyme closer to the electrode surface due to its oxi-reduction facility.
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Martins, M.V.A. (2022). Direct and Mediated Electron Transfer in Enzyme Electrodes. In: Crespilho, F.N. (eds) Advances in Bioelectrochemistry Volume 1. Springer, Cham. https://doi.org/10.1007/978-3-030-94988-4_2
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