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Kinetic chemiluminescence as a method for study of free radical reactions

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Abstract

The review gives concepts of analysis of kinetics of complex reactions with the participation of free radicals. The concepts are based on the comparison of kinetic curves of chemiluminescent reactions in the presence of a physical enhancer coumarin C-525 with the simulated kinetic functions. This method was applied to the investigation of the mechanism of a branched-chain reaction of lipid peroxidation in biological and phospholipid membranes as well as to the effect of antioxidants and determination of their activities. It was also used for studying the reaction of formation of free radicals in the complex of cytochrome c with cardiolipin. This reaction plays a key role in the initiation of apoptosis.

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Correspondence to Y. A. Vladimirov.

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Original Russian Text © Y.A. Vladimirov, E.V. Proskurnina, D.Yu. Izmajlov, 2011, published in Biofizika, 2011, Vol. 56, No. 6, pp. 1081–1090.

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Vladimirov, Y.A., Proskurnina, E.V. & Izmajlov, D.Y. Kinetic chemiluminescence as a method for study of free radical reactions. BIOPHYSICS 56, 1055–1062 (2011). https://doi.org/10.1134/S0006350911060200

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  • DOI: https://doi.org/10.1134/S0006350911060200

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