Abstract
The resting membrane potential of isolated hepatocytes from 2- and 20-month-old rats and its changes upon activation of cells by adrenaline have been studied by the method of quantitative microfluorimetry using diethyl derivatives of polymethine probes (H-510 and D-307). The potential was estimated by the Nernst equation adapted to lipophilic cationic probes. It was shown using both probes that the transmembrane potential of hepatocytes decreases with age. The microfluorimetry data were confirmed by the results of spectrofluorimetric measurements in a cell suspension. Changes in fluorescence occurring in adrenaline-activated single cells and suspensions were unidirectional, the effect of the hormone on the cells of old animals being less pronounced. The results indicate that the potential of the plasma membrane of individual hepatocytes can be adequately estimated by microfluorimetry, which can be used in metabolic and toxicologic investigations.
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References
C. D. Bortner, M. Gomez-Angelats, and J. A. Cidlowski, Biol. Chem. 276, 4304 (2001).
C. Mann and J. A. Cidlowski, Endocrinology 142, 421 (2001).
C. L. Deckers, A. B. Lyons, K. Samuel, et al., Exp. Cell Res. 208, 342 (1993).
J. Witkowski and H. S. Mickem, Immunology 56, 307 (1985).
V. V. Frolkis, S. A. Tanin, E. N. Gorban, et al., ZFA 42, 77 (1987).
T. M. Hagen, D. L. Yowe, J. C. Bartholomew, et al., Proc. Natl. Acad. Sci. USA 94, 3064 (1997).
H. M. Shapiro, Methods 21, 271 (2000).
F. Wang, W. B. Tan, Y. Zhang, et al., Nanotechnology 17, R1 (2006).
T. K. Das, N. Periasamy, and G. Krishnamoorthy, Biophys. 64, 1122 (1993).
A. Waggoner, Membr. Biol. 27, 317 (1976).
K. E. Ekerman and N. E. Saris, Biochim. Biophys. Acta 426, 624 (1976).
P. C. Laris, D. F. Bahr, and R. R. Chaffe, Biochim. Biophys. Acta 376, 415 (1975).
M. Kataoka, Y. Fukura, Y. Shinohara, and Y. Baba, Electrophoresis 26, 3025 (2005).
B. E. Seligmann and J. I. Gallin, Cell. Physiol. 115, 105 (1983).
B. Ehrenberg and V. Montana, Biophys. 53, 785 (1988).
F. I. Kiprianov and G. P. Pilyugin, Ucheni Zap. Kharkiv. Un-tu 10, 91 (1937).
I. P. Kanaeva, A. V. Karyakin, T. V. Alenicheva, et al., Tsitologiya 17, 545 (1975).
B. I. Shapiro, Usp. Khimii 75, 484 (2006).
M. Terasaki, J. Song, J. R. Wong, et al., Cell 38, 101 (1984).
T. Sawanobori, H. Takanashi, M. Hiraoka, et al., Cell. Physiology 139, 580 (1989).
V. V. Frolkis, Probl. Endokrinol. 23, 86 (1977).
O. H. Peterson, J. Physiol. (London) 239, 647 (1974).
S. J. Weiss and J. W. Putney, J. Pharmacol. Exp.Ther. 207, 669 (1978).
J. Graf and O. H. Peterson, J. Physiol. (London) 284, 105 (1978).
S. M. Lee and M.G. Clemens, Am. J. Physiol. Gastrointest. Liver Physiol. 263, G319 (1992).
D. Kessel, W. Beck, D. Kukuruga, and V. Schulz, Cancer Research 51, 4665 (1991).
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Original Russian Text © Yu.V. Malyukin, N.S. Kavok, I.A. Borovoi, A.M. Stepanenko, M.Yu. Malyukina, P.A. Petrukhin, 2011, published in Biofizika, 2011, Vol. 56, No. 3, pp. 475–480.
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Malyukin, Y.V., Kavok, N.S., Borovoi, I.A. et al. A study of the effect of adrenaline on the transmembrane potential of the plasma membrane of hepatocytes from rats of different age using fluorescent probes. BIOPHYSICS 56, 452–456 (2011). https://doi.org/10.1134/S000635091103016X
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DOI: https://doi.org/10.1134/S000635091103016X