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Study of the reaction of kidney collecting duct principal cells to hypotonic shock. Experiment and mathematical model

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Abstract

The reaction of rat kidney collecting duct principal cells to hypotonic shock was studied. The changes in cell relative volume were measured using fluorescent dye calcein, and a mathematical model based on our experimental results was developed. It was shown that regulatory volume decrease is mainly provided by significant release of osmolytes from the cell and decrease of the plasma membrane water permeability. Using our model, we calculated the membrane water permeability and found it to decrease from 2 · 10−1 to 2 · 10−2 cm/s. We conclude that for effective RVD to occur, a dramatic increase in the membrane permeability to K+, Cl− and organic anions is necessary.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    A. V. Ilyaskin, G. S. Baturina & E. I. Solenov

  2. Lavrent’ev Institute of Hydrodynamics. Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    D. A. Medvedev & A. P. Ershov

  3. Novosibirsk State University, Novosibirsk, 630090, Russia

    D. A. Medvedev, A. P. Ershov & E. I. Solenov

Authors
  1. A. V. Ilyaskin
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  2. G. S. Baturina
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  3. D. A. Medvedev
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  4. A. P. Ershov
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  5. E. I. Solenov
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Correspondence to E. I. Solenov.

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Original Russian Text © A.V. Ilyaskin, G.S. Baturina, D.A. Medvedev, A.P. Ershov, E.I. Solenov, 2011, published in Biofizika, 2011, Vol. 56, No. 3, pp. 550–560.

Translation of the text provided by the authors; some redaction imposed for literacy and comprehensibility.

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Ilyaskin, A.V., Baturina, G.S., Medvedev, D.A. et al. Study of the reaction of kidney collecting duct principal cells to hypotonic shock. Experiment and mathematical model. BIOPHYSICS 56, 516–524 (2011). https://doi.org/10.1134/S0006350911030092

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  • DOI: https://doi.org/10.1134/S0006350911030092

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