Skip to main content
SpringerLink
Log in

Participation of adenylate kinase in the regulation of chloroplast energy exchange

  • Cell Biophysics
  • Published:
Biophysics Aims and scope Submit manuscript

Abstract

The influence of adenylate kinase on the rates of glucose-6-phosphate synthesis and ferricyanide reduction in a system containing chloroplasts, hexokinase, and ADP at low concentration during photophos-phorylation has been studied. It has been found that the addition of adenylate kinase into the reaction medium under phosphorylation results in a simultaneous increase in the rate of ferricyanide reduction and glucose-6-phosphate synthesis. In this case, the ratio of glucose-6-phosphate formed to the quantity of ferricyanide reduced was close to unity as the concentration of adenylate kinase in the medium increased. The concentrations of glucose-6-phosphate and ferricyanide reduced in the system sharply increased with time; at the same time, no significant decrease in ADP concentration and AMP accumulation by the methods available was found. Hence, the limiting factors in these reactions are not the concentrations but the rates of diffusion of the substrates. Presumably, diffusion limitations in the system are eliminated owing to the participation of adenylate kinase. The results obtained are discussed in terms of the model according to which the regulation of the diffusion of adenine nucleotides and the control of regeneration of ATP according to its requirements in correlation with other regulation mechanisms can occur in chloroplasts upon adenylate kinase functioning by direct and reverse connection of the shuttle type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. E. Shnol’, Physicochemical Factors of Biological Evolution (Nauka, Moscow, 1979) [in Russian].

    Google Scholar 

  2. S. E. Shnol’, E. A. Ermakova, and G. M. Frank, in Methodological and Theoretical Problems of Biophysics (Nauka, Moscow, 1979), pp. 90–98 [in Russian].

    Google Scholar 

  3. I. V. Berezin and S. D. Varfolomeev, Biokinetics (Nauka, Moscow, 1979) [in Russian].

    Google Scholar 

  4. I. M. Kartashov, A. D. Makarov, and A. N. Mal’yan, Biofizika 18(2), 273 (1973).

    Google Scholar 

  5. I. M. Kartashov, Candidate’s Dissertation in Biology (1979).

  6. C. Aflalo and N. Shavit, FEBS Lett. 154(1), 175 (1983).

    Article  Google Scholar 

  7. C. Aflalo and N. Shavit, Curr. Topics Cell. Regul. Enzyme Catal. Contr. 24, 435 (1984).

    Google Scholar 

  8. Y. Mukohata, T. Yagi, Y. Sugiyama, et al., I. Bioenergetics 7(3), 91 (1975).

    Article  Google Scholar 

  9. V. A. Saks, Y. O. Belikova, and A. V. Kuznetsov, Biochim. Biophys. Àcta 1074, 302 (1991).

    Google Scholar 

  10. M. Wyss, J. Smeitiuk, R. A. Wevers, and T. Wallimann, Biochim. Biophys. Acta 1102, 119 (1992).

    Article  Google Scholar 

  11. P. Dzeja, A. Kalvenas, A. Toleikis, and A. Praskevicius, Biochem. Int. 10, 259 (1985).

    Google Scholar 

  12. P. P. Dzheya, A. A. Kal’venas, A. I. Toleikis, et al., Biokhimiya 51(6), 974 (1986).

    Google Scholar 

  13. N. Ya. Kholodenko, I. M. Kartashov, and A. D. Makarov, Biokhimiya 48(3), 471 (1983).

    Google Scholar 

  14. I. M. Kartashov, N. Ya. Kholodenko, and A. D. Makarov, Biokhimiya 49(4), 556 (1984).

    Google Scholar 

  15. I. M. Kartashov, N. Ya. Kholodenko, and A. D. Makarov, Biokhimiya 50, 447 (1985).

    Google Scholar 

  16. G. R. Schacterle and R. L. Pollack, Analyt. Biochem. 51(2), 654 (1973).

    Article  Google Scholar 

  17. D. I. Arnon, Plant Physiol. 24(1), 1 (1949).

    Article  Google Scholar 

  18. F. Whatley and D. I. Arnon, in Methods in Enzymology (Acad Press, N.Y.-L., 1963), vol. 6, pp. 308–313.

    Book  Google Scholar 

  19. A. D. Vinogradov, Biokhimiya 64(11), 1443 (1999).

    Google Scholar 

  20. S. Robinson and J. T. Wiskich, Plant Physiol. 58(2), 156 (1976).

    Article  Google Scholar 

  21. D. L. Purich and H. J. Fromm, J. Biolog. Chem. 248, 461 (1973).

    Google Scholar 

  22. P. Horton, in Topics in Photosynhesis. Photosynthetic Mechanisms and the Environment (1985), vol. 6, pp. 135–188.

    Google Scholar 

  23. J. Edwards and D. Walker, Photosynthesis of C3 and C4 Plants: Mechanisms and Regulation (Mir, Moscow, 1986).

    Google Scholar 

  24. A. N. Mal’yan, Doctoral Dissertation in Biology (1988).

  25. V. P. Skulachev, Energetics of Biological Membranes (Nauka, Moscow, 1989) [in Russian].

    Google Scholar 

  26. T. Bizouarn, I. J. Kouchkovsky, and F. Haraux, Biochim. Biophys. Acta 974(1), 104 (1989)

    Article  Google Scholar 

  27. P. D. Boyer, Biochim. Biophys. Acta 1140(1), 215 (1993).

    MathSciNet  ADS  Google Scholar 

  28. A. N. Malyan, Sov. Sci. Rev. D. Physicochem. Biol. 12(4), 1 (1994).

    Google Scholar 

  29. N. I. Kobozev, in Catalysis in Higher School (Izd. MGU, Moscow, 1962), pp. 43–56 [in Russian].

    Google Scholar 

  30. V. I. Deshcherevsky, A. M. Zhabotinsky, E. E. Selkov, et al., Biofizika 15(2), 225 (1970).

    Google Scholar 

  31. L. A. Blyumenfeld, Problems of Biological Physics (Nauka, Moscow, 1977) [in Russian].

    Google Scholar 

  32. D. S. Chernavsky, Yu. I. Khurgin, and S. E. Shnol’, Biofizika 27(5), 775 (1987).

    Google Scholar 

  33. I. I. Shmal’gauzen, Cybernetic Questions of Biology (Nauka, Novosibirsk, 1968) [in Russian].

    Google Scholar 

  34. M. V. Vol’kenshtein, Biofizika 15(8), 215 (1970).

    Google Scholar 

  35. L. Noda, in The Enzymes (Acad. Press, New York-London, 1973), pp. 279–305.

    Google Scholar 

  36. I. M. Kartashov, Abstracts of Papers, II All-Russia Congress of Photobiologists (Pushchino, 1998), pp. 45–47.

  37. Y. Kobayashi, Y. Inoue, F. Furuya, et al., Planta 147, 69 (1979).

    Article  Google Scholar 

  38. M. N. Kondrashova, Biofizika 24(3), 450 (1989).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    I. M. Kartashov

Authors
  1. I. M. Kartashov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. M. Kartashov.

Additional information

Original Russian Text © I.M. Kartashov, 2009, published in Biofizika, 2009, Vol. 54, No. 5, pp. 841–845.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kartashov, I.M. Participation of adenylate kinase in the regulation of chloroplast energy exchange. BIOPHYSICS 54, 593–597 (2009). https://doi.org/10.1134/S0006350909050078

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006350909050078

Key words

Search

Navigation