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Influence of cholesterol on the formation of palmitate/Ca2+-activated pores in mitochondria and liposomes

  • Cell Biophysics
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Abstract

The influence of cholesterol on the formation of a mitochondrial cyclosporin A-insensitive palmitate/Ca2+-activated pore has been studied. Loading of mitochondrial membranes with cholesterol increases the rate of mitochondrial swelling induced by palmitic acid (≥20 µM) and Ca2+ (30 µM). This effect is not related to changes in the functional activity of organelles, since cholesterol does not influence the mitochondrial respiration in different metabolic states. At the same time, palmitate/Ca2+-induced permeabilization of azolectin/cholesterol liposomes is more pronounced than that of azolectin liposomes. In the liposomal membrane, Ca2+ induces phase separation of palmitic acid into distinct membrane domains; the presence of cholesterol in membranes enhances this effect.

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Abbreviations

CsA:

cyclosporin A

NAO:

nonyl acridine orange

Pal:

palmitic acid

PalCaP:

palmitate/Ca2+-activated pore

SRB:

sulforhodamine B

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    N. V. Belosludtseva, K. N. Belosludtsev, A. V. Agafonov & G. D. Mironova

  2. Pushchino State University, Pushchino, Moscow Region, 142290, Russia

    G. D. Mironova

Authors
  1. N. V. Belosludtseva
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  2. K. N. Belosludtsev
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  3. A. V. Agafonov
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  4. G. D. Mironova
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Correspondence to N. V. Belosludtseva.

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Original Russian Text © N.V. Belosludtseva, K.N. Belosludtsev, A.V. Agafonov, G.D. Mironova, 2009, published in Biofizika, 2009, Vol. 54, No. 3, pp. 464–470.

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Belosludtseva, N.V., Belosludtsev, K.N., Agafonov, A.V. et al. Influence of cholesterol on the formation of palmitate/Ca2+-activated pores in mitochondria and liposomes. BIOPHYSICS 54, 323–326 (2009). https://doi.org/10.1134/S0006350909030117

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  • DOI: https://doi.org/10.1134/S0006350909030117

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