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A comparative analysis of the contracture responses induced by acetylcholine and choline in the twitch and tonic fibers of frog skeletal muscles

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Abstract

A comparative analysis of the contractile responses induced by acetylcholine and replacement of the external Na+ ions with choline ions in the isolated twitch and tonic fibers of frog skeletal muscles was performed. The effects of extracellular Ca2+ concentration and several pharmacological agents modulating the activity of various systems maintaining Ca2+ level in the myoplasm (dantrolene, cresol, d-tubocurarine, and tetrodotoxin) were studied. It has been found that a voltage-dependent Ca2+ release from the sarcoplasmic reticulum depot is the main mechanism inducing the acetylcholine contracture in the fibers of both types. However, the twitch and tonic fibers differ in the properties of the α-isoform and(or) the ratio of α- to β-isoforms of ryanodine-sensitive channels. In the fibers of both types, the replacement of over 25% of Na+ ions with choline induces long-term contracture responses, which are also mediated by activation of acetylcholine receptors. It is assumed that an additional mechanism—accumulation of choline ions in the myoplasm and their direct action on the ryanodine-sensitive channels—is involved in the development of such contractile responses.

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Abbreviations

SR:

sarcoplasmic reticulum

RyRs:

ryanodine-sensitive receptors/channels

DICR:

depolarization-induced Ca2+ release from SR

CICR:

Ca-induced Ca2+ release from SR

SC:

single contraction

ACh:

acetylcholine chloride

AChC:

acetylcholine contracture

ChC:

choline-induced contracture

nAChR:

nicotinic acetylcholine receptor

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Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Toreza 44, St. Petersburg, 194223, Russia

    I. E. Katina & G. A. Nasledov

Authors
  1. I. E. Katina
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  2. G. A. Nasledov
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Correspondence to I. E. Katina.

Additional information

Original Russian Text © I.E. Katina, G.A. Nasledov, 2008, published in Biofizika, 2008, Vol. 53, No. 6, pp. 1078–1086.

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Katina, I.E., Nasledov, G.A. A comparative analysis of the contracture responses induced by acetylcholine and choline in the twitch and tonic fibers of frog skeletal muscles. BIOPHYSICS 53, 608–614 (2008). https://doi.org/10.1134/S0006350908060262

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  • DOI: https://doi.org/10.1134/S0006350908060262

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