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Decrease in the electrogenic Contribution of Na,K-ATPase and the resting membrane potential as a possible mechanism of Ca2+ accumulation in rat soleus muscle in a short-term gravity unloading

  • Complex Systems Biophysics
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Abstract

The resting membrane potential and electrogenic contribution of α1- and α2-isoforms of Na+/K+-ATPase in the rat soleus muscle at early stages of gravity unloading were analyzed. The role of L-type calcium channels in accumulation of calcium ions in the myoplasm under these conditions was estimated. After 3-day antiorthostatic suspension, the resting membrane potential of the muscle fibers decreased from −71.0 ± 0.5 to −66.8 ± 0.7 mV, the muscle excitability reduced, and a trend of muscle fatigue acceleration appeared. The electrogenic contribution of ouabain-sensitive α2-isoform of Na+/K+-ATPase, determined as the depolarization caused by 1μM ouabain, decreased after suspension from 6.2 ± 0.6 to 0.5 ± 0.8 mV. The contribution of ouabain-resistant α1-isoform of Na+/K+-ATPase, determined as an additional depolarization after addition of 500 μM ouabain, decreased from 4.6 ± 0.6 to 2.6 ± 0.6 mV. The intensity of Fluo-4AM fluorescence in individual muscle fibers increased after suspension more than fourfold, which suggests an elevated calcium concentration in the myoplasm. A local delivery of nifedipine, a blocker of the L-type calcium channels, to the muscle removed this effect. The existence of a selective mechanism suppressing the electrogenic contribution of Na+/K+-ATPase α2-isoform, which is the main cause of the muscle fiber membrane depolarization after 3-day suspension, is postulated. The depolarization can activate part of potential-sensitive L-type Ca2+ channels, causing the accumulation of calcium ions in the muscle fiber myoplasm.

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Authors and Affiliations

  1. St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia

    I. I. Krivoi, V. V. Kravtsova, I. V. Kubasov, A. V. Prokof’ev & T. M. Drabkina

  2. Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe sh. 76a, Moscow, 123007, Russia

    E. G. Altaeva & B. S. Shenkman

  3. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111, Russia

    E. E. Nikol’sky

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  1. I. I. Krivoi
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  2. V. V. Kravtsova
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  3. E. G. Altaeva
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  4. I. V. Kubasov
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  5. A. V. Prokof’ev
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  6. T. M. Drabkina
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  7. E. E. Nikol’sky
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  8. B. S. Shenkman
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Original Russian Text © I.I. Krivoi, V.V. Kravtsova, E.G. Altaeva, I.V. Kubasov, A.V. Prokof’ev, T.M. Drabkina, E.E. Nikol’sky, B.S. Shenkman, 2008, published in Biofizika, 2008, Vol. 53, No. 6, pp. 1051–1057.

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Krivoi, I.I., Kravtsova, V.V., Altaeva, E.G. et al. Decrease in the electrogenic Contribution of Na,K-ATPase and the resting membrane potential as a possible mechanism of Ca2+ accumulation in rat soleus muscle in a short-term gravity unloading. BIOPHYSICS 53, 586–591 (2008). https://doi.org/10.1134/S0006350908060225

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  • DOI: https://doi.org/10.1134/S0006350908060225

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