Abstract
Encapsulation of enzymes (lactate dehydrogenase and urease) in polyelectrolyte shells was assessed with a view to designing enzymic microdiagnostics for low-molecular compounds in native biological fluids. Polyelectrolyte microcapsules were prepared with two polyanions [poly(styrenesulfonate) PSS and dextran sulfate DS] and two polycations [poly(allylamine) PAA and poly(diallyldimethylammonium) PDADMA]; calcium carbonate microspherulites with embedded enzymes served as “cores.” It was demonstrated that the main problem in making such a biosensor is to select a pair of oppositely charged polyelectrolytes that would be optimal for enzyme functioning. The best pairs were PAA/DS and PAA/PSS for lactate dehydrogenase, and PSS/PAA and PSS/PDADMA for urease. We designed and prepared enzyme-containing microcapsules differing in polyelectrolyte composition and number of layers, and investigated their properties.
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Original Russian Text © B.I. Sukhorukov, S.A. Tikhonenko, E. A. Saburova, A.V. Dubrovskii, Yu.N. Dybovskaya, L.I. Shabarchina, 2007, published in Biofizika, 2007, Vol. 52, No. 6, pp. 1041–1048.
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Sukhorukov, B.I., Tikhonenko, S.A., Saburova, E.A. et al. Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics. BIOPHYSICS 52, 575–581 (2007). https://doi.org/10.1134/S0006350907060085
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DOI: https://doi.org/10.1134/S0006350907060085