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The study of a fluorescent biosensor based on polyelectrolyte microcapsules with encapsulated glucose oxidase

  • Biophysical Chemistry
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Abstract

A fluorescent biosensor is synthesized and described. The biosensor consists of polyelectrolyte microcapsules with glucose oxidase (GOx) entrapped in the cavities and an oxygen-sensitive fluorescent indicator Ru(dpp) immobilized in shells, where Ru(dpp) is tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride. The theoretical activity of the encapsulated GOx and the effect storage time and medium composition have on the stability of sensor microcapsules are determined from polarographic measurements. No change in the activity of the encapsulated enzyme and or its loss to the storage medium are detected over the test period. The dispersion medium (water or a phosphate buffer) are shown to have no effect on the activity of microcapsules with immobilized GOx. The described optical sensor could be used as an alternative to electrochemical sensors for in vitro determination of glucose in the clinically important range of concentrations (up to 10 mmol/L).

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    L. I. Kazakova, N. P. Sirota, T. V. Sirota & L. I. Shabarchina

Authors
  1. L. I. Kazakova
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  2. N. P. Sirota
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  3. T. V. Sirota
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  4. L. I. Shabarchina
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Correspondence to L. I. Kazakova.

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Original Russian Text © L.I. Kazakova, N.P. Sirota, T.V. Sirota, L.I. Shabarchina, 2017, published in Zhurnal Fizicheskoi Khimii, 2017, Vol. 91, No. 9, pp. 1613–1618.

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Kazakova, L.I., Sirota, N.P., Sirota, T.V. et al. The study of a fluorescent biosensor based on polyelectrolyte microcapsules with encapsulated glucose oxidase. Russ. J. Phys. Chem. 91, 1828–1832 (2017). https://doi.org/10.1134/S0036024417090151

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  • DOI: https://doi.org/10.1134/S0036024417090151

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