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Influence of light-induced changes in stromal and lumenal pH on electron transport kinetics in chloroplasts: Mathematical modeling

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Abstract

A mathematical model of electron and proton transport in higher plant chloroplasts is built with allowance for light-induced pH changes in the stroma and the thylakoid lumen. The model considers the key steps of electron transport from photosystem II to NADP+, the terminal acceptor of PS I, ATP synthesis from ADP and inorganic phosphate coupled with transmembrane proton transport, consumption of NADPH and ATP in the Calvin cycle. Account of the influence of lumenal and stromal pH changes on the rate of electron transport at the plastoquinone segment of the chain and on the acceptor side of PS I allows description of the particular features of the complex kinetics of electron transport in intact chloroplasts. Calculations show that accelerated efflux of electrons at the acceptor part of PS I, resulting from activation of the Calvin cycle reaction upon light-induced stromal alkalization, may be one of the causes of the multiphasic kinetics of the redox conversions of the PS I primary donor P700, which is characteristic of dark-adapted cyanobacteria, algae, and intact higher plant chloroplasts.

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Authors and Affiliations

  1. Physical Faculty, Moscow State University, Moscow, 119992, Russia

    A. E. Frolov & A. N. Tikhonov

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  1. A. E. Frolov
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  2. A. N. Tikhonov
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Correspondence to A. N. Tikhonov.

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Original Russian Text © A.E. Frolov, A.N. Tikhonov, 2007, published in Biofizika, 2007, Vol. 52, No. 4, pp. 656–666.

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Frolov, A.E., Tikhonov, A.N. Influence of light-induced changes in stromal and lumenal pH on electron transport kinetics in chloroplasts: Mathematical modeling. BIOPHYSICS 52, 398–405 (2007). https://doi.org/10.1134/S0006350907040070

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