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Application of a photosystem II model for analysis of fluorescence induction curves in the 100 ns to 10 s time domain after excitation with a saturating light pulse

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Abstract

A mathematical model of photosystem II (PSII) events was used to analyze chlorophyll fluorescence transients in the time domain from 100 ns to 10 s after excitation with a saturating 10-ns flash, applied as a part of specialized illumination protocol, using preparations of a thermophilic strain of the unicellular green alga, Chlorella pyrenoidosa Chick (using both intact and diuron-treated cells). Analysis of simulation results has proven that particular attention should be given to flash-induced recombination processes, including nonradiative recombination in PSII, while subsequent charge transfer along the electron transport chain of thylakoid membrane can be adequately described by a single reaction of quinone reoxidation. The PSII model was extended by taking inhibition by diuron of the electron transport in the acceptor side of PSII into account, which allowed simulation of fluorescence induction curves observed in the presence of this inhibitor. The model parameters were determined (stromal pH, rate constants of nonradiative recombination, and the initial reduction state of the quinone pool) which provided adequate simulation of experimentally observed ratios of the maximal and initial fluorescence levels (F m/F 0).

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Abbreviations

Chl:

chlorophyll of the antenna and the reaction centers

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

ETC:

electron transport chain

FIC:

fluorescence induction curves

Fl:

chlorophyll fluorescence

H +L :

protons of the thylakoid lumen

H +S :

protons of the chloroplast stroma

OEC:

oxygen-evolving complex

P680:

chlorophyll of photosystem II reaction center

Phe:

pheophytin

PSII:

photosystem II

QA and QB :

primary and secondary quinone acceptors of PSII

RC:

reaction center

YZ :

redox-active tyrosine of PSII

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Biology, Moscow State University, Moscow, 119992, Russia

    N. E. Belyaeva, V. Z. Pashchenko, G. Yu. Riznichenko & A. B. Rubin

  2. Max-Volmer Institute, Technical University, Berlin, 10623, Germany

    G. Renger

Authors
  1. N. E. Belyaeva
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  2. V. Z. Pashchenko
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  3. G. Renger
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  4. G. Yu. Riznichenko
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  5. A. B. Rubin
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Original Russian Text © N.E. Belyaeva, V.Z. Pashchenko, G. Renger, G.Yu. Riznichenko, A.B. Rubin, 2006, published in Biofizika, 2006, Vol. 51, No. 6, pp. 976–990.

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Belyaeva, N.E., Pashchenko, V.Z., Renger, G. et al. Application of a photosystem II model for analysis of fluorescence induction curves in the 100 ns to 10 s time domain after excitation with a saturating light pulse. BIOPHYSICS 51, 860–872 (2006). https://doi.org/10.1134/S0006350906060030

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  • DOI: https://doi.org/10.1134/S0006350906060030

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