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LH2 Complex from Sulfur Bacteria Allochromatium vinosum – Natural Singlet Oxygen Sensor

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Abstract

It was established that in a heterogeneous model system, which consisted of two types of complexes: reaction center or core complex of photosystem 2 of higher plants and LH2 complex of the sulfur bacterium Alc. vinosum, BChl850 oxidation of the LH2 complex could be observed under illumination by the light at a wavelength of 662 nm, which is the red absorption band of Chl. It has been shown that this process induces release of singlet oxygen, which is generated in photosystem II complexes and then partially diffuses into LH2 complex, where it oxidizes BChl850. It was established by HPLC that this results in formation of a product of BChl oxidation, 3-acetylchlorophyll. The process of BChl850 oxidation is inhibited by singlet oxygen quenchers (Trolox and Na ascorbate). It is suggested that the LH2 complex from the sulfur bacterium Alc. vinosum could be used to detect generation of singlet oxygen by the chlorophyll containing samples.

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Abbreviations

AcChl:

3-acetyl-chlorophyll

BChl:

bacteriochlorophyll

Chl:

chlorophyll

LH1 and LH2:

light-harvesting complexes

PSII:

photosystem II

RC:

reaction center

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Acknowledgments

The authors are grateful to Z. A. Zhuravleva (Institute of Basic Biological Problems, Russian Academy of Sciences) for help in growing bacterial cultures.

Funding

This work was financially supported by the State Budget project no. 122041100204-3.

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    Zoya K. Makhneva, Tatyana N. Smolova, Maksim A. Bolshakov & Andrey A. Moskalenko

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  1. Zoya K. Makhneva
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  2. Tatyana N. Smolova
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  3. Maksim A. Bolshakov
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  4. Andrey A. Moskalenko
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Contributions

A. A. Moskalenko – concept of the study and supervision of the work; Z. K. Makhneva, T. N. Smolova, M. A. Bolshakov – conducting experiments; Z. K. Makhneva, T. N. Smolova, M. A. Bolshakov, A. A. Moskalenko – discussion of the results of the study; A. A. Moskalenko – writing text of the paper; M. A. Bolshakov – editing of text of the paper.

Corresponding author

Correspondence to Maksim A. Bolshakov.

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The authors declare no conflict of interests in financial or any other sphere. This article does not contain any studies with human participants or animals performed by any of the authors.

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Makhneva, Z.K., Smolova, T.N., Bolshakov, M.A. et al. LH2 Complex from Sulfur Bacteria Allochromatium vinosum – Natural Singlet Oxygen Sensor. Biochemistry Moscow 87, 1159–1168 (2022). https://doi.org/10.1134/S0006297922100091

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