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Modern Methods for Assessment of microRNAs

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Abstract

The review discusses modern methods for the quantitative and semi-quantitative analysis of miRNAs, which are small non-coding RNAs affecting numerous biological processes such as development, differentiation, metabolism, and immune response. miRNAs are considered as promising biomarkers in the diagnosis of various diseases.

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Abbreviations

CEAM:

cyclic enzymatic amplification method

CRISPR/Cas:

clustered regularly interspaced palindromic repeats/CRISPR associated protein

CHA:

catalytic hairpin assembly

EXPAR:

exponential amplification reaction

HCR:

hybridization chain reaction

HRCA:

hyperbranched rolling circle amplification

ICSDP:

isothermal circular-strand-displacement polymerization

LAMP:

loop-mediated isothermal amplification

PCR:

polymerase chain reaction

RCA:

rolling circle amplification

SEXPAR:

symmetric exponential amplification reaction

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The work was supported by the Russian Foundation for Basic Research (project no. 21-54-53007).

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  1. Lomonosov Moscow State University, Faculty of Chemistry, 119991, Moscow, Russia

    Oleg L. Bodulev & Ivan Yu. Sakharov

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  1. Oleg L. Bodulev
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Correspondence to Ivan Yu. Sakharov.

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Bodulev, O.L., Sakharov, I.Y. Modern Methods for Assessment of microRNAs. Biochemistry Moscow 87, 425–442 (2022). https://doi.org/10.1134/S0006297922050042

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