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CRISPR/Cas Genome Editing in Filamentous Fungi

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Abstract

The review describes the CRISPR/CAS system and its adaptation for the genome editing in filamentous fungi commonly used for production of enzyme complexes, enzymes, secondary metabolites, and other compounds used in industrial biotechnology and agriculture. In the second part of this review, examples of the CRISPR/CAS technology application for improving properties of the industrial strains of fungi from the Trichoderma, Aspergillus, Penicillium, and other genera are presented. Particular attention is given to the efficiency of genome editing, as well as system optimization for specific industrial producers.

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Abbreviations

CRISPR/Cas:

clustered regularly interspaced short palindromic repeats and CRISPR-associated protein

DSB:

double-strand break

HDV:

hepatitis D virus

HH:

hammerhead

HR:

homologous recombination

NHEJ:

non-homologous end joining

NLS:

nuclear localization sequence

sgRNA:

single guide RNA

tracrRNA:

trans-activating CRISPR RNA

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This work was partially supported by the Russian Foundation for Basic Research (project no. 18-29-07070).

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  1. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071, Moscow, Russia

    Aleksandra M. Rozhkova & Valeriy Yu. Kislitsin

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Rozhkova, A.M., Kislitsin, V.Y. CRISPR/Cas Genome Editing in Filamentous Fungi. Biochemistry Moscow 86 (Suppl 1), S120–S139 (2021). https://doi.org/10.1134/S0006297921140091

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