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Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3

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Abstract

Yanbian yellow cattle are one of the top five largest breeds of cattle in China. We had previously found that bta-miR-1271 is differentially expressed in the longissimus dorsi muscles of Yanbian yellow bulls and steers. However, whether bta-miR-1271 affects bovine fat formation is unclear. In this study, we used target gene prediction, dual-luciferase reporter assay, and transfection-mediated overexpression and inhibition of bta-miR-1271 in a culture of Yanbian yellow cattle preadipocytes to investigate the role of bta-miR-1271 in adipogenesis. We showed that bta-miR-1271 directly targets the 3′-untranslated region (3′-UTR) of the activating transcription factor 3 (ATF3) mRNA and downregulates its expression. Overexpression of bta-miR-1271 enforced by the miRNA mimics promoted triglyceride accumulation and significantly upregulated expression of the adipogenic peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) genes at both the protein and mRNA levels, as demonstrated by RT-qPCR and Western blot analyses. Conversely, inhibition of bta-miR-1271 expression produced the opposite effect. Our results show that bta-miR-1271 regulates differentiation of Yanbian yellow cattle preadipocytes by inhibiting ATF3 expression, which highlights the importance of microRNA-mediated regulation of adipogenesis. miR-1271 and its target gene(s) may provide a new research direction for investigating biological agents affecting intramuscular fat deposition in cattle.

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Abbreviations

ATF3:

activation transcription factor 3

C/EBPα:

CCAAT/enhancer binding protein α

miRNA:

microRNA

MUT:

mutant type

NC:

negative control

PPARγ:

peroxisome proliferator-activated receptor γ

UTR:

untranslated region

WT:

wild type

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Funding

This study was supported by the Science and Technology Development Plan of Jilin Province of China (project 20160204017NY).

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    Authors and Affiliations

    1. Agriculture College, Yanbian University, 133002, Yanji, Jilin, China

      H. Y. Xu, J. Shao, B. Z. Yin, L. M. Zhang, J. S. Zhang & G. J. Xia

    2. Faculty of Agriculture and Life Science, Hirosaki University, 036-8560, Hirosaki, Japan

      J. C. Fang

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    1. H. Y. Xu
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    Correspondence to G. J. Xia.

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    Xu, H., Shao, J., Yin, B. et al. Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3. Biochemistry Moscow 85, 749–757 (2020). https://doi.org/10.1134/S0006297920070032

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