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Essential Light Chains of Myosin and Their Role in Functioning of the Myosin Motor

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Abstract

This review summarizes current data on the structure and functions of myosin essential light chains (ELCs) and on their role in functioning of the myosin head as a molecular motor. The data on structural and functional features of the N-terminal extension of myosin ELC from skeletal and cardiac muscles are analyzed; the role of this extension in the ATP- dependent interaction of myosin heads with actin in the molecular mechanism of muscle contraction is discussed. The data on possible interactions of the ELC N-terminal extension with the myosin head motor domain in the myosin ATPase cycle are presented, including the results of the authors’ studies that are in favor of such interactions.

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Abbreviations

A1 and A2:

isoforms of skeletal muscle myosin essential (alkali) light chains

AlF –4 :

aluminum fluoride anion

BeFx :

beryllium fluoride anion

DLS:

dynamic light scattering

DSC:

differential scanning calorimetry

ELC:

myosin essential light chain

ELCa:

atrial ELC

ELCv:

ventricular ELC

FHC:

familial hypertrophic cardiomyopathy

FRET:

Forster resonance energy transfer

MM :

molecular mass

RLC:

myosin regulatory light chain

S1:

myosin subfragment 1

S1(A1) and S1(A2):

myosin subfragment 1 isoforms containing alkali light chains A1 and A2, respectively

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Federal Research Center “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071, Moscow, Russia

    D. S. Logvinova & D. I. Levitsky

  2. Lomonosov Moscow State University, Faculty of Biology, 119991, Moscow, Russia

    D. S. Logvinova

  3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    D. I. Levitsky

Authors
  1. D. S. Logvinova
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  2. D. I. Levitsky
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Correspondence to D. S. Logvinova.

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Original Russian Text © D. S. Logvinova, D. I. Levitsky, 2018, published in Biokhimiya, 2018, Vol. 83, No. 8, pp. 1190–1210.

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Logvinova, D.S., Levitsky, D.I. Essential Light Chains of Myosin and Their Role in Functioning of the Myosin Motor. Biochemistry Moscow 83, 944–960 (2018). https://doi.org/10.1134/S0006297918080060

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  • DOI: https://doi.org/10.1134/S0006297918080060

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