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O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104

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Abstract

Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.

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Abbreviations

bp:

base pair

COSY:

correlation spectroscopy

GC:

gas chromatography

Gro:

glycerol

HMBC:

heteronuclear multiple-bond correlation

HSQC:

heteronuclear single quantum correlation

OPS:

O-specific polysaccharide

ROESY:

rotating-frame Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

Und-P:

undecaprenyl phosphate

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    E. L. Zdorovenko, A. S. Shashkov, O. G. Ovchinnikova & Y. A. Knirel

  2. TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457, Tianjin, China

    Y. Wang, T. Chen & B. Liu

  3. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312, Moscow, Russia

    A. K. Golomidova & A. V. Letarov

  4. Federal Research and Clinical Centre of Physico-Chemical Medicine, 119435, Moscow, Russia

    V. V. Babenko

Authors
  1. E. L. Zdorovenko
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  2. Y. Wang
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  3. A. S. Shashkov
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  4. T. Chen
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  5. O. G. Ovchinnikova
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  6. B. Liu
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  7. A. K. Golomidova
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  8. V. V. Babenko
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  9. A. V. Letarov
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  10. Y. A. Knirel
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Corresponding author

Correspondence to Y. A. Knirel.

Additional information

Published in Russian in Biokhimiya, 2018, Vol. 83, No. 5, pp. 703–711.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM17-453, January 29, 2018.

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Zdorovenko, E.L., Wang, Y., Shashkov, A.S. et al. O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104. Biochemistry Moscow 83, 534–541 (2018). https://doi.org/10.1134/S0006297918050061

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  • DOI: https://doi.org/10.1134/S0006297918050061

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