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Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid

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Abstract

Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Galp-(1→6)-β-D-Galp-(1→3)-D-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Galp-(1→3)-DGalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.

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Abbreviations

COSY:

correlation spectroscopy

CPS:

capsular polysaccharide

HMBC:

heteronuclear multiple-bond correlation

HSQC:

heteronuclear single-quantum coherence

Pse:

pseudaminic acid

RHb:

(R)-3-hydroxybutanoyl

ROESY:

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. A. Kasimova, N. P. Arbatsky, A. S. Shashkov & Yu. A. Knirel

  2. Higher Chemical College of the Russian Academy of Sciences, Dmitry Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

    A. A. Kasimova

  3. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997, Moscow, Russia

    M. M. Shneider & K. A. Miroshnikov

  4. Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow Region, Russia

    A. V. Popova

  5. State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia

    A. V. Popova

  6. Department of Clinical Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu, India

    Veeraraghavan Balaji

  7. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, 66160, USA

    Indranil Biswas

Authors
  1. A. A. Kasimova
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  2. M. M. Shneider
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  3. N. P. Arbatsky
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  4. A. V. Popova
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  5. A. S. Shashkov
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  6. K. A. Miroshnikov
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  7. Veeraraghavan Balaji
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  8. Indranil Biswas
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  9. Yu. A. Knirel
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Corresponding author

Correspondence to Yu. A. Knirel.

Additional information

Published in Russian in Biokhimiya, 2017, Vol. 82, No. 4, pp. 655-663.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM16-342, December 5, 2016.

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Kasimova, A.A., Shneider, M.M., Arbatsky, N.P. et al. Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid. Biochemistry Moscow 82, 483–489 (2017). https://doi.org/10.1134/S0006297917040101

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  • DOI: https://doi.org/10.1134/S0006297917040101

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