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Structure of the K82 Capsular Polysaccharide from Acinetobacter baumannii LUH5534 Containing a d-Galactose 4,6-Pyruvic Acid Acetal

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Abstract

Type K82 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii LUH5534. The structure of a linear tetrasaccharide repeating unit of the CPS was established by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. Proteins encoded by the KL82 capsule gene cluster in the genome of LUH5534 were assigned to roles in the synthesis of the K82 CPS. In particular, functions were assigned to two new glycosyltransferases (Gtr152 and Gtr153) and a novel pyruvyltransferase, Ptr5, responsible for the synthesis of D-galactose 4,6-(R)-pyruvic acid acetal.

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Abbreviations

COSY:

correlation spectroscopy

CPS:

capsular polysaccharide

HMBC:

heteronuclear multiple-bond correlation

HSQC:

heteronuclear single-quantum coherence

ROESY:

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

Und-P:

undecaprenyl phosphate

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. A. Kasimova, N. P. Arbatsky, A. S. Shashkov & Y. A. Knirel

  2. Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

    A. A. Kasimova

  3. School of Molecular Bioscience, The University of Sydney, Sydney, NSW, 2006, Australia

    J. J. Kenyon & R. M. Hall

  4. Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD, 4059, Australia

    J. J. Kenyon

  5. Moscow Institute of Physics and Technology, 141700, Dolgoprudny, Moscow Region, Russia

    A. V. Popova

  6. State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia

    A. V. Popova

  7. School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia

    R. M. Hall

Authors
  1. A. A. Kasimova
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  2. J. J. Kenyon
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  3. N. P. Arbatsky
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  4. A. S. Shashkov
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  5. A. V. Popova
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  6. Y. A. Knirel
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  7. R. M. Hall
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Corresponding author

Correspondence to A. A. Kasimova.

Additional information

Published in Russian in Biokhimiya, 2018, Vol. 83, No. 7, pp. 1061–1066.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM18-021, April 2, 2018.

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Kasimova, A.A., Kenyon, J.J., Arbatsky, N.P. et al. Structure of the K82 Capsular Polysaccharide from Acinetobacter baumannii LUH5534 Containing a d-Galactose 4,6-Pyruvic Acid Acetal. Biochemistry Moscow 83, 831–835 (2018). https://doi.org/10.1134/S0006297918070064

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  • DOI: https://doi.org/10.1134/S0006297918070064

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