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“Suppressor factor” of neutrophils: A short story of a long-term misconception

  • Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
  • Published:
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Abstract

A large body of evidence obtained during the last decade has demonstrated that neutrophils suppress T cell proliferation in different models of inflammation and cell interaction. The commonly used method for assessing cell proliferation and proliferation inhibition is measuring [3H]thymidine incorporation into cells. Earlier, we observed inhibition of [3H]thymidine uptake in experiments on neutrophil-mediated regulation of T cell response in tuberculosis immunity. Here, we used different types of proliferating cells to analyze the nature of the soluble “neutrophil factor” by a variety of methods (dialysis, HPLC, mass spectrometry, and NMR) and unambiguously demonstrated that neutrophils do not synthesize a specific factor inhibiting cell proliferation, but secrete high concentrations of extracellular thymidine that competitively inhibit [3H]thymidine incorporation. Although the physiological significance of thymidine secretion by neutrophils remains unknown, this phenomenon should be carefully considered when designing test systems for studying cell–cell interactions.

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Abbreviations

APCs:

antigen-presenting cells

CFSE:

carboxyfluorescein succinimidyl ester

13C-1H-HSQC:

heteronuclear single quantum correlation spectroscopy

DQF-COSY:

double-quantum filtered correlation spectroscopy

MHC:

major histocompatibility complex

PBS:

phosphate buffered saline

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Authors and Affiliations

  1. Central Research Institute for Tuberculosis, 107564, Moscow, Russia

    I. A. Linge, E. V. Kondratieva, T. K. Kondratieva & A. S. Apt

  2. Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991, Moscow, Russia

    V. A. Makarov

  3. Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy, Lomonosov Moscow State University, 119991, Moscow, Russia

    V. I. Polshakov & O. Yu. Savelyev

Authors
  1. I. A. Linge
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  2. E. V. Kondratieva
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  3. T. K. Kondratieva
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  4. V. A. Makarov
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  5. V. I. Polshakov
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  6. O. Yu. Savelyev
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  7. A. S. Apt
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Corresponding authors

Correspondence to I. A. Linge or A. S. Apt.

Additional information

Original Russian Text © I. A. Linge, E. V. Kondratieva, T. K. Kondratieva, V. A. Makarov, V. I. Polshakov, O. Yu. Savelyev, A. S. Apt, 2016, published in Biokhimiya, 2016, Vol. 81, No. 11, pp. 1530–1539.

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Linge, I.A., Kondratieva, E.V., Kondratieva, T.K. et al. “Suppressor factor” of neutrophils: A short story of a long-term misconception. Biochemistry Moscow 81, 1284–1292 (2016). https://doi.org/10.1134/S0006297916110067

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  • DOI: https://doi.org/10.1134/S0006297916110067

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