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Chemical modification of photosystem II core complex pigments with sodium borohydride

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Abstract

The reaction of the irreversible chemical reduction of the 131-keto C=O group of pheophytin a (Pheo a) with sodium borohydride in reaction centers (RCs) of functionally active spinach photosystem II (PS II) core complexes was studied. Stable, chromatographically purified PS II core complex preparations with altered chromophore composition are obtained in which ∼25% of Pheo a molecules are modified to 131-deoxo-131-hydroxy-Pheo a. Some of the chlorophyll a molecules in the complexes were also irreversibly reduced with borohydride to 131-deoxo-131-hydroxy-chlorophyll a. Based on the results of comparative study of spectral, biochemical, and photochemical properties of NaBH4-treated and control preparations, it was concluded that: (i) the borohydride treatment did not result in significant dissociation of the PS II core complex protein ensemble; (ii) the modified complexes retained the ability to photoaccumulate the radical anion of the pheophytin electron acceptor in the presence of exogenous electron donor; (iii) only the photochemically inactive pheo-phytin PheoD2 is subjected to the borohydride treatment; (iv) the Qx optical transition of the PheoD2 molecule in the RC of PS II core complexes is located at 543 nm; (v) in the Qy spectral region, PheoD2 probably absorbs at ∼680 nm.

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Abbreviations

ΔA :

absorbance changes

Car:

β-carotene

Chl:

chlorophyll

Cyt b559:

cytochrome b559

P680 :

dimer of Chl a molecules in photosystem II reaction center

Pheo:

pheo-phytin

PheoD1 and PheoD2 :

Pheo bound to D1 and D2 polypeptides, respectively

PS II:

photosystem II

QA :

primary quinone acceptor

RC:

reaction center

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Authors and Affiliations

  1. Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, 142290, Pushchino, Moscow Region, Russia

    M. I. Vishnev, A. A. Zabelin, V. A. Shkuropatova, M. F. Yanyushin, V. A. Shuvalov & A. Ya. Shkuropatov

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  1. M. I. Vishnev
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  2. A. A. Zabelin
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  3. V. A. Shkuropatova
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  4. M. F. Yanyushin
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  5. V. A. Shuvalov
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  6. A. Ya. Shkuropatov
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Correspondence to A. Ya. Shkuropatov.

Additional information

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 4, pp. 492–500.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM12-310, February 17, 2013.

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Vishnev, M.I., Zabelin, A.A., Shkuropatova, V.A. et al. Chemical modification of photosystem II core complex pigments with sodium borohydride. Biochemistry Moscow 78, 377–384 (2013). https://doi.org/10.1134/S0006297913040068

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