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Catalytic properties and amino acid sequence of endo-1→3-β-D-glucanase from the marine mollusk Tapes literata

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Abstract

A specific 1→3-β-D-glucanase with molecular mass 37 kDa was isolated in homogeneous state from crystalline style of the commercial marine mollusk Tapes literata. It exhibits maximal activity within the pH range from 4.5 to 7.5 at 45dgC. The 1→3-β-D-glucanase catalyzes hydrolysis of β-1→3 bonds in glucans as an endoenzyme with retention of bond configuration, and it has transglycosylating activity. The K m for hydrolysis of laminaran is 0.25 mg/ml. The enzyme is classified as a glucan endo-(1→3)-β-D-glucosidase (EC 3.2.1.39). The cDNA encoding this 1→3-β-D-glucanase from T. literata was sequenced, and the amino acid sequence of the enzyme was determined. The endo-1→3-β-D-glucanase from T. literata was assigned to the 16th structural family (GHF 16) of O-glycoside hydrolases.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100 let Vladivostoku 159, 690022, Vladivostok, Russia

    A. M. Zakharenko, M. I. Kusaykin, S. N. Kovalchuk, V. V. Sova, A. S. Silchenko, S. D. Anastyuk, V. A. Rasskazov & T. N. Zvyagintseva

  2. Far Eastern Federal University, ul. Sukhanova 8, 690950, Vladivostok, Russia

    A. A. Belik

  3. Nha Trang Institute of Technology Research and Application, Hung Vuong st. 02, Nha Trang, Vietnam

    Bui Minh Ly

Authors
  1. A. M. Zakharenko
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  2. M. I. Kusaykin
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  3. S. N. Kovalchuk
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  4. V. V. Sova
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  5. A. S. Silchenko
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  6. A. A. Belik
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  7. S. D. Anastyuk
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  8. Bui Minh Ly
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  9. V. A. Rasskazov
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  10. T. N. Zvyagintseva
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Correspondence to A. M. Zakharenko.

Additional information

Original Russian Text © A. M. Zakharenko, M. I. Kusaykin, S. N. Kovalchuk, V. V. Sova, A. S. Silchenko, A. A. Belik, S. D. Anastyuk, Bui Minh Ly, V. A. Rasskazov, T. N. Zvyagintseva, 2012, published in Biokhimiya, 2012, Vol. 77, No. 8, pp. 1067–1079.

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Zakharenko, A.M., Kusaykin, M.I., Kovalchuk, S.N. et al. Catalytic properties and amino acid sequence of endo-1→3-β-D-glucanase from the marine mollusk Tapes literata . Biochemistry Moscow 77, 878–888 (2012). https://doi.org/10.1134/S0006297912080081

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  • DOI: https://doi.org/10.1134/S0006297912080081

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