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Programmed cell death in plants: Protective effect of mitochondrial-targeted quinones

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Abstract

Ubiquinone or plastoquinone covalently linked to synthetic decyltriphenylphosphonium (DTPP+) or rhodamine cations prevent programmed cell death (PCD) in pea leaf epidermis induced by chitosan or CN−. PCD was monitored by recording the destruction of cell nuclei. CN− induced the destruction of nuclei in both epidermal cells (EC) and guard cells (GC), whereas chitosan destroyed nuclei in EC not in GC. The half-maximum concentrations for the protective effects of the quinone derivatives were within the pico- and nanomolar range. The protective effect of the quinones was removed by a protonophoric uncoupler and reduced by tetraphenylphosphonium cations. CN−-Induced PCD was accelerated by the tested quinone derivatives at concentrations above 10−8–10−7 M. Unlike plastoquinone linked to the rhodamine cation (SkQR1), DTPP+ derivatives of quinones suppressed menadione-induced H2O2 generation in the cells. The CN−-induced destruction of GC nuclei was prevented by DTPP+ derivatives in the dark not in the light. SkQR1 inhibited this process both in the dark and in the light, and its effect in the light was similar to that of rhodamine 6G. The data on the protective effect of cationic quinone derivatives indicate that mitochondria are involved in PCD in plants.

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Abbreviations

te]BQ:

p-benzoquinone

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

DAPI:

4′,6-diamidino-2-phenylindole dihydrochloride

DCF:

2′,7′-dichlorofluorescein

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

DTPP+ :

decyltriphenylphosphonium cation

EC:

basic epidermal cells

GC:

stoma guard cells

MitoQ:

10-(6′-ubiquinonyl)decyltriphenylphosphonium

NBT:

nitroblue tetrazolium

PCD:

programmed cell death

ROS:

reactive oxygen species

SkQ1:

10-(6′-plastoquinonyl)decyltriphenylphosphonium

SkQ3:

10-(6′-methylplastoquinonyl)decyltriphenylphosphonium

SkQR1:

10-(plastoquinonyl)decylrhodamine 19

TPP+ :

tetraphenylphosphonium cation

Δyy:

transmembrane electrical potential difference

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    L. A. Vasil’ev, E. V. Dzyubinskaya, D. B. Kiselevsky, A. A. Shestak & V. D. Samuilov

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  1. L. A. Vasil’ev
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  2. E. V. Dzyubinskaya
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  3. D. B. Kiselevsky
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  5. V. D. Samuilov
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Correspondence to V. D. Samuilov.

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Original Russian Text © L. A. Vasil’ev, E. V. Dzyubinskaya, D. B. Kiselevsky, A. A. Shestak, V. D. Samuilov, 2011, published in Biokhimiya, 2011, Vol. 76, No. 10, pp. 1374–1386.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM11-124, September 4, 2011.

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Vasil’ev, L.A., Dzyubinskaya, E.V., Kiselevsky, D.B. et al. Programmed cell death in plants: Protective effect of mitochondrial-targeted quinones. Biochemistry Moscow 76, 1120–1130 (2011). https://doi.org/10.1134/S0006297911100051

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