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Regulatory protein Yap1 is involved in response of yeast Saccharomyces cerevisiae to nitrosative stress

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Abstract

The goal of this work was to investigate the possible involvement of protein transcription factor Yap1 in regulation of activity of antioxidant enzymes superoxide dismutase and catalase during yeast response to nitrosative stress. It was found that the inactivation of the YAP1 gene, encoding Yap1p, cancelled the activation of superoxide dismutase and catalase by NO-donors. Then, using chimeric protein Yap1-GFP, we found the accumulation of Yap1p in the nucleus in response to nitrosative stress. Therefore, we conclude that these results in combination with previous data clearly demonstrate the involvement of Yap1p in upregulation of superoxide dismutase and catalase in yeast cells in response to nitrosative stress.

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Abbreviations

GFP:

green fluorescent protein

G6PDH:

glucose-6-phosphate dehydrogenase

GR:

glutathione reductase

GSH/GSSG:

reduced/oxidized glutathione

GSNO:

S-nitrosoglutathione

IDH:

isocitrate dehydrogenase

(i)NOS:

(inducible) NO-synthase

MDH:

malate dehydrogenase

ROS (RNS):

reactive oxygen (nitrogen) species

SNP:

sodium nitro-prusside

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, ul. Shevchenko 57, 76025, Ivano-Frankivsk, Ukraine

    O. V. Lushchak & V. I. Lushchak

  2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto, Japan

    Y. Inoue

Authors
  1. O. V. Lushchak
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  2. Y. Inoue
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  3. V. I. Lushchak
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Correspondence to V. I. Lushchak.

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Published in Russian in Biokhimiya, 2010, Vol. 75, No. 5, pp. 735–743.

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Lushchak, O.V., Inoue, Y. & Lushchak, V.I. Regulatory protein Yap1 is involved in response of yeast Saccharomyces cerevisiae to nitrosative stress. Biochemistry Moscow 75, 629–635 (2010). https://doi.org/10.1134/S0006297910050135

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  • DOI: https://doi.org/10.1134/S0006297910050135

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