Abstract
The coherent 11-cis-retinal photoisomerization dynamics in bovine rhodopsin was studied by femtosecond time-resolved laser absorption spectroscopy at 30-fs resolution. Femtosecond pulses of 500, 535, and 560 nm wavelength were used for rhodopsin excitation to produce different initial Franck-Condon states and relevant distinct values of the vibrational energy of the molecule in its electron excited state. Time evolution of the photoinduced rhodopsin absorption spectra was monitored after femtosecond excitation in the spectral range of 400–720 nm. Oscillations of the time-resolved absorption signals of rhodopsin photoproducts represented by photorhodopsin570 with vibrationally-excited all-trans-retinal and rhodopsin498 in its initial state with vibrationally-excited 11-cis-retinal were studied. These oscillations reflect the dynamics of coherent vibrational wave-packets in the ground state of photoproducts. Fourier analysis of these oscillatory components has revealed frequencies, amplitudes, and initial phases of different vibrational modes, along which the motion of wave-packets of both photoproducts occurs. The main vibrational modes established are 62, 160 cm−1 and 44, 142 cm−1 for photorhodopsin570 and for rhodopsin498, respectively. These vibrational modes are directly involved in the coherent reaction under the study, and their amplitudes in the power spectrum obtained through the Fourier transform of the kinetic curves depend on the excitation wavelength of rhodopsin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- batho535 :
-
bathorhodopsin
- FC:
-
Franck-Condon
- HTG:
-
n-nonyl-β-D-heptylthioglucoside
- PES:
-
potential energy surface
- photo570 :
-
photorhodopsin
- R498 :
-
rhodopsin
- ROS:
-
rod outer segments
References
Lamb, T. D., Collin, S. P., and Pugh, E. N. (2007) Nat. Rev. Neurosci., 8, 960–976.
Schoenlein, R. W., Peteanu, L. A., Mathies, R. A., and Shank, C. V. (1991) Science. New Ser., 254, 412–415.
Peteanu, L. A., Schoenlein, R. W., Wang, Q., Mathies, R. A., and Shank, C. V. (1993) Proc. Natl. Acad. Sci. USA, 90, 11762–11766.
Haran, G., Morlino, E. A., Matthes, J., Callender, R. H., and Hochstrasser, R. M. J. (1999) Phys. Chem. A, 103, 2202–2207.
Kandori, H., Katsuta, Y., Msayoshi, I., and Sasabe, H. (1995) J. Am. Chem. Soc., 117, 2669–2670.
Kim, J. E., Tauber, M. J., and Mathies, R. A. (2001) Biochemistry, 40, 13774–13778.
Koyama, Y., Kubo, K., Komori, M., Yasuda, H., and Mukai, Y. (1991) Photochem. Photobiol., 54, 433–443.
Cooper, A. (1979) Nature, 282, 531–533.
Shichida, Y., Matuoka, S., and Yoshizawa, T. (1984) Photobiochem. Photobiophys., 7, 221–228.
Mizukami, T., Kandori, H., Shichida, Y., Chen, A.-H., Derguini, F., Caldwell, C. G., Bigge, C., Nakanishi, K., and Yoshizawa, T. (1993) Proc. Natl. Acad. Sci. USA, 90, 4072–4076.
Wang, Q., Schoenlein, R. W., Peteanu, L. A., Mathies, R. A., and Shank, C. V. (1994) Science, New Ser., 266, 422–424.
Smitienko, O. A., Shelaev, I. V., Gostev, F. E., Fel’dman, T. B., Nadtochenko, V. A., Sarkisov, O. M., and Ostrovsky, M. A. (2008) Dokl. RAN, 421, 1–5.
Kochendoerfer, G. G., and Mathies, R. A. (1996) J. Phys. Chem., 100, 14526–14532.
Garavelli, M., Celani, P., Bernardi, F., Robb, M. A., and Olivucci, M. (1997) J. Am. Chem. Soc., 119, 6891–6901.
Garavelli, M., Vreven, T., Celani, P., Bernardi, F., Robb, M. A., and Olivucci, M. (1998) J. Am. Chem. Soc., 120, 1285–1288.
Gonzalez-Luque, R., Garavelli, M., Bernardi, F., Merchan, M., Robb, M. A., and Olivucci, M. (2000) Proc. Natl. Acad. Sci. USA, 97, 9379–9384.
Worth, G. A., and Cederbaum, L. S. (2004) Annu. Rev. Phys. Chem., 55, 127–158.
Hudson, B. S., Kohler, B. E., and Schulten, K. (1982) in Excited States (Lim, E. C., ed.) Vol. 6, Academic Press, N. Y., pp. 1–95.
Loppnow, G. R., and Mathies, R. A. (1988) Biophys. J., 54, 35–43.
Lin, S. W., Groesbeek, M., van der Hoef, I., Verdegem, P., Lugtenburg, J., and Mathies, R. A. (1998) J. Phys. Chem. B, 102, 2787–2806.
Kukura, P., McCamant, D. W., Yoon, S., Wandschneider, D. B., and Mathies, R. A. (2005) Science, 310, 1006–1009.
Antipin, S. A., Fel’dman, T. B., Gostev, F. E., Smitienko, O. A., Sarkisov, O. M., and Ostrovsky, M. A. (2004) Dokl. RAN, 396, 105–108.
Okada, T., Takeda, K., and Kouyama, T. (1998) Photochem. Photobiol., 67, 495–499.
Shelaev, I. V., Gostev, F. E., Nadtochenko, V. A., Shkuropatov, A. Ya., Zabelin, A. A., Mamedov, M. D., Semenov, A. Yu., Sarkisov, O. M., and Shuvalov, V. A. (2008) Photosynth. Res., 98, 95–103.
Hou, B., Friedman, N., Ottolenghi, M., Sheves, M., and Ruhman, S. (2003) Chem. Phys. Lett., 381, 549–555.
Kim, J. E., and Mathies, R. A. (2002) J. Phys. Chem. A, 106, 8508–8515.
Prokhorenko, V. I., Nagy, A. M., Waschuk, S. A., Brown, L. S., Birge, R. R., and Miller, R. J. D. (2006) Science, 313, 1257–1261.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O. A. Smitienko, M. N. Mozgovaya, I. V. Shelaev, F. E. Gostev, T. B. Feldman, V. A. Nadtochenko, O. M. Sarkisov, M. A. Ostrovsky, 2010, published in Biokhimiya, 2010, Vol. 75, No. 1, pp. 34–45.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM09-105, December 20, 2009.
Rights and permissions
About this article
Cite this article
Smitienko, O.A., Mozgovaya, M.N., Shelaev, I.V. et al. Femtosecond formation dynamics of primary photoproducts of visual pigment rhodopsin. Biochemistry Moscow 75, 25–35 (2010). https://doi.org/10.1134/S0006297910010049
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297910010049