Abstract
Nonaqueous titration was used for detection of free amino groups in the polymeric matrix of plant cell walls. The content of amino groups varied in the range 0.54–0.91 and total nitrogen in the range 1.0–4.2 mmol per gram dry mass of cell walls depending on the plant species. However, these data on the high content of free amino groups do not correlate with the present day concept that the nitrogen fraction in charged amino groups in plant cell wall proteins, which are assumed to be mainly amino groups of lysine and arginine residues, is about 10%. It is supposed that most detected free amino groups belong to the hydroxy-amino acids hydroxyproline and tyrosine that can be bound at the hydroxyl group with the carbohydrate part of glycoprotein or another structural cell wall polymer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fry, S. C. (2004) New Phytologist, 161, 641–675.
Gorshkova, T. A. (2007) Plant Cell Wall as Dynamic System [in Russian], Nauka, Moscow.
Zeier, J., Goll, A., Yokoyama, M., Karahara, I., and Schreiber, L. (1999) Plant, Cell Environ., 22, 271–279.
Shiga, T. M., Lajolo, F. V., and Filisetti, T. M. C. C. (2003) Cienc. Technol. Aliment., 23, 141–148.
Gassab, G. I. (1998) Annu. Rev. Plant Physiol. Plant Mol. Biol., 49, 281–309.
Gassab, G. I., and Varner, J. E. (1988) Annu. Rev. Plant Physiol. Plant Mol. Biol., 39, 321–353.
Feiz, L., Irshad, M., Pont-Lezica, R. F., Canut, H., and Jamet, E. (2006) Plant Method, 2, 10–22.
Jamet, E., Canut, H., Boudart, G., and Pont-Lezica, R. F. (2006) Trends Plant Sci., 11, 33–39.
Sharova, E. I. (2004) in Plant Cell Wall, St. Petersburg State University Publishing, St. Petersburg.
Baslavskaya, S. S., and Trubetskova (1964) in Practical Course on Plant Physiology [in Russian], Moscow State University Publishing, Moscow.
Robinson, S. P., and Dountov, S. D. (1985) Austr. J. Plant Physiol., 12, 471–479.
Meychik, N. R., Ermakov, I. P., and Savvateeva, M. V. (1999) Plant Physiol. (Moscow), 46, 742–747.
Meychik, N. R., and Yermakov, I. P. (1999) Plant & Soil, 217, 257–264.
Meychik, N. R., Matveeva, N. P., Nikolaeva, Yu. I., Chaikova, A. V., and Yermakov, I. P. (2006) Biochemistry (Moscow), 71, 893–899.
Cheronis, N. D., and Ma, T. S. (1973) in Micro- and Semimicromethods of Organic Functional Analysis [Russian translation], Khimiya, Moscow.
Feofilova, E. P. (1983) in Cell Wall of Fungi [in Russian], Nauka, Moscow.
Talmadge, K. W., Keegstra, K., Bauer, W. D., and Albersheim, P. (1973) Plant Physiol., 51, 158–173.
Richter, C., and Dainty, J. (1989) Can. J. Bot., 67, 460–465.
Brady, J. D., Sadler, I. H., and Fry, S. C. (1996) Biochem. J., 315, 323–327.
Brady, J. D., Sadler, I. H., and Fry, S. C. (1998) Phytochemistry, 47, 349–353.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N. R. Meychik, Yu. I. Nikolaeva, I. P. Ermakov, 2009, published in Biokhimiya, 2009, Vol. 74, No. 8, pp. 1145–1151.
Rights and permissions
About this article
Cite this article
Meychik, N.R., Nikolaeva, Y.I. & Ermakov, I.P. Nonaqueous titration of amino groups in polymeric matrix of plant cell walls. Biochemistry Moscow 74, 933–937 (2009). https://doi.org/10.1134/S0006297909080161
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297909080161