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Design of a novel interleukin-13 antagonist from analysis of informational structure

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Abstract

Interleukin-13 (IL-13) is one of the cytokines involved in the development of Th2-type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL-13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF-1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full-length IL-13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL-13.

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Abbreviations

ANIS:

analysis of informational structure

DV:

deletion variant

ELIS:

elements of informational structure

IDIC:

increased degree of information coordination

IDIC-sites:

sites with a locally increased degree of information coordination between residues

IL-13:

interleukin-13

IPTG:

isopropyl-β-D-thiogalactoside

IS:

informational structure

IU:

informational units

SOE:

splice overlap extension

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    A. N. Nekrasov, L. E. Petrovskaya, V. A. Toporova, E. A. Kryukova & M. P. Kirpichnikov

  2. Department of Health Care, Moscow Research Institute of Medical Ecology, Simferopolsky Bulvar 8, 117638, Moscow, Russia

    A. V. Rodina & E. Yu. Moskaleva

Authors
  1. A. N. Nekrasov
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  2. L. E. Petrovskaya
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  3. V. A. Toporova
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  4. E. A. Kryukova
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  5. A. V. Rodina
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  6. E. Yu. Moskaleva
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  7. M. P. Kirpichnikov
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Corresponding author

Correspondence to A. N. Nekrasov.

Additional information

Published in Russian in Biokhimiya, 2009, Vol. 74, No. 4, pp. 493–500.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-256, February 1, 2009.

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Nekrasov, A.N., Petrovskaya, L.E., Toporova, V.A. et al. Design of a novel interleukin-13 antagonist from analysis of informational structure. Biochemistry Moscow 74, 399–405 (2009). https://doi.org/10.1134/S0006297909040075

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  • DOI: https://doi.org/10.1134/S0006297909040075

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