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Antibodies against DNA hydrolyze DNA and RNA

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Abstract

In this work, rabbits were immunized with a high polymer DNA complexed with methylated BSA (mBSA) and by mBSA. It is shown that electrophoretically homogeneous preparations of polyclonal antibodies (Ab) from non-immunized rabbits and animals immunized by mBSA do not exhibit catalytic activity. Ab from the blood of rabbits immunized with the DNA-mBSA complex hydrolyzed poly(C) and different RNAs with efficiency exceeding that towards DNA by approximately 3–4 orders of magnitude. Affinity chromatography of the IgG on DNA cellulose separated the Ab into fractions hydrolyzing both RNA and DNA, and for the first time fractions that hydrolyze only RNA were found. Kinetic parameters that characterize the RNA and DNA hydrolysis by initial Ab preparations and their fractions obtained by separation on an affinity sorbent are compared.

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Abbreviations

Ab:

antibodies

AID:

autoimmune diseases

mBSA:

methylated BSA

pAb:

polyclonal Ab

pIgG:

polyclonal immunoglobulin G

scDNA and rDNA:

supercoiled and relaxed DNA, respectively

SLE:

systemic lupus erythematosus

VIP:

vasoactive intestinal polypeptide

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, ul. Akademika Lavrentieva 8, 630090, Novosibirsk, Russia

    M. A. Krasnorutskii, V. N. Buneva & G. A. Nevinsky

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  1. M. A. Krasnorutskii
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  2. V. N. Buneva
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  3. G. A. Nevinsky
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Correspondence to G. A. Nevinsky.

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Original Russian Text © M. A. Krasnorutskii, V. N. Buneva, G. A. Nevinsky, 2008, published in Biokhimiya, 2008, Vol. 73, No. 11, pp. 1547–1560.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-028, October 12, 2008.

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Krasnorutskii, M.A., Buneva, V.N. & Nevinsky, G.A. Antibodies against DNA hydrolyze DNA and RNA. Biochemistry Moscow 73, 1242–1253 (2008). https://doi.org/10.1134/S0006297908110114

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  • DOI: https://doi.org/10.1134/S0006297908110114

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