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Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site

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Non-Natural Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1973))

Abstract

Methods for the preparation of DNA duplexes containing interstrand covalent cross-links may facilitate research in the fields of biochemistry, molecular biology, nanotechnology, and materials science. Here we report methods for the synthesis and isolation of DNA duplexes containing a site-specific, chemically stable, reduced covalent interstrand cross-link between a guanine residue and an abasic site. The method uses experimental techniques and equipment that are common in most biochemical laboratories and inexpensive, commercially available oligonucleotides and reagents.

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Acknowledgment

We are grateful to the National Institutes of Health for supporting this work (ES021007).

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Authors and Affiliations

  1. Department of Chemistry, University of Missouri, Columbia, MO, USA

    Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang & Kent S. Gates

  2. Department of Biochemistry, University of Missouri, Columbia, MO, USA

    Kent S. Gates

  3. Department of Chemistry, University of California Riverside, Riverside, CA, USA

    Nathan E. Price & Yinsheng Wang

Authors
  1. Maryam Imani Nejad
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  2. Xu Guo
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  3. Kurt Housh
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  4. Christopher Nel
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  5. Zhiyu Yang
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  6. Nathan E. Price
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  7. Yinsheng Wang
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  8. Kent S. Gates
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Corresponding author

Correspondence to Kent S. Gates .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA

    Nathaniel Shank

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Nejad, M.I. et al. (2019). Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site. In: Shank, N. (eds) Non-Natural Nucleic Acids. Methods in Molecular Biology, vol 1973. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9216-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9216-4_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9215-7

  • Online ISBN: 978-1-4939-9216-4

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