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Ribonuclease, deoxyribonuclease, and antiviral activity of Escherichia coli-expressed Bougainvillea xbuttiana antiviral protein 1

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Abstract

A full-length cDNA encoding ribosome-inactivating/antiviral protein from the leaves of Bougainvillea xbuttiana was recently isolated. The coding region of cDNA was cloned and expressed in Escherichia coli, and the protein product was designated as BBAP1 (Bougainvillea xbuttiana antiviral protein 1). BBAP1 showed ribonuclease activity against Torula yeast RNA. It also exhibited depurination activity against supercoiled pBlueScript SK+ plasmid DNA in a concentration dependent manner, and was found to convert nicked circular DNA into linear form only at higher concentration. On bioassay, BBAP1 exhibited antiviral activity against sunnhemp rosette virus infecting Cyamopsis tetragonoloba leaves in which 95% inhibition of local lesion formation was observed.

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Abbreviations

AVP:

antiviral protein

BBAP1:

Bougainvillea xbuttiana antiviral protein 1

ORF:

open reading frame

PAP:

pokeweed antiviral protein

RIP:

ribosome-inactivating protein

SRV:

sunnhemp rosette virus

TMV:

tobacco mosaic virus

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Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012, India

    N. L. Choudhary, O. P. Yadav & M. L. Lodha

  2. Emeritus Scientist, Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India

    M. L. Lodha

Authors
  1. N. L. Choudhary
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  2. O. P. Yadav
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  3. M. L. Lodha
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Corresponding author

Correspondence to M. L. Lodha.

Additional information

Published in Russian in Biokhimiya, 2008, Vol. 73, No. 3, pp. 336–341.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM07-272, December 16, 2007.

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Choudhary, N.L., Yadav, O.P. & Lodha, M.L. Ribonuclease, deoxyribonuclease, and antiviral activity of Escherichia coli-expressed Bougainvillea xbuttiana antiviral protein 1. Biochemistry Moscow 73, 273–277 (2008). https://doi.org/10.1134/S000629790803005X

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  • DOI: https://doi.org/10.1134/S000629790803005X

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