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Ribosome-inactivating proteins in plant biology

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Fig. 1

Abbreviations

PAP:

Pokeweed antiviral protein

RIP:

Ribosome-inactivating protein

SOD:

Superoxide dismutase

References

  • Barbieri L, Ferreras JM, Barraco A, Ricci P, Stirpe F (1992) Some ribosome-inactivating proteins depurinate ribosomal RNA at multiple sites. Biochem J 286:1–4

    CAS  PubMed  Google Scholar 

  • Barbieri L, Valbonesi P, Bonora E, Gorini P, Bolognesi A, Stirpe F (1997) Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A). Nucleic Acids Res 25:518–522

    Article  CAS  PubMed  Google Scholar 

  • Barbieri L, Brigotti M, Perocco P, Carnicelli D, Ciani M, Mercatali L, Stirpe F (2003) Ribosome-inactivating proteins depurinate poly(ADP-ribosyl)ated poly(ADP-ribose) polymerase and have transforming activity for 3T3 fibroblasts. FEBS Lett 538:178–182

    Article  CAS  PubMed  Google Scholar 

  • Day PJ, Lord JM, Roberts LM (1998) The deoxyribonuclease activity attributed to ribosome-inactivating proteins is due to contamination. Eur J Biochem 258:540–545

    Article  CAS  PubMed  Google Scholar 

  • He W-J, Liu WY (2004) Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA. Biochem J 377:17–23

    Google Scholar 

  • Helmy M, Lombard S, Pieroni G (1999) Ricin RCA60: evidence of its phospholipase activity. Biochem Biophys Res Commun 258:252–255

    Article  CAS  PubMed  Google Scholar 

  • Lam YH, Wong YS, Wang B, Wong RNS, Yeung HW, Shaw PC (1996) Use of trichosanthin to reduce infection by turnip mosaic virus. Plant Sci 114:11–117

    Article  Google Scholar 

  • Li X-D, Chen W-F, Liu W-Y, Wang G-H (1997) Large-scale preparation of two new ribosome-inactivating proteins, Cinnamomin and Camphorin, from the seeds of Cinnamomum camphora. Protein Expr Purif 10:27–31

    Article  CAS  PubMed  Google Scholar 

  • Lodge JK, Kaniewski WK, Tumer NE (1993) Broad-spectrum virus resistance in transgenic plants expressing pokeweed antiviral protein. Proc Natl Acad Sci USA 90:7089–7093

    CAS  PubMed  Google Scholar 

  • Moon YH, Song SK, Choi KW, Lee JS (1997) Expression of a cDNA encoding Phytolacca insularis antiviral protein confers virus resistance on transgenic potato plants. Mol Cells 7:807–815

    CAS  PubMed  Google Scholar 

  • Ng TB, Lam YW, Wang H (2003) Calcaelin, a new protein with translation-inhibiting, antiproliferative and antimitogenic activities from mosaic puffball mushroom Calvatia caelata. Planta Med 69:212–217

    Article  CAS  PubMed  Google Scholar 

  • Parikh BA, Coetzer C, Tumer NE (2002) Pokeweed antiviral protein regulates the stability of its own mRNA by a mechanism that requires depurination but can be separated from depurination of the α-sarcin/ricin loop of rRNA. J Biol Chem 277:41428–41437

    Article  CAS  PubMed  Google Scholar 

  • Park S-W, Stevens NM, Vivanco JM (2002) Enzymatic specificity of three ribosome-inactivating proteins against fungal ribosomes, and correlation with antifungal activity. Planta 216:227–234

    Article  CAS  PubMed  Google Scholar 

  • Park, S-W, Vepachedu R, Owens RA, Vivanco JM (2004) The N-glycosidase activity of ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs. J Biol Chem 279:34165–34174

    Google Scholar 

  • Rajamohan F, Venkatachalam TK, Irvin JD, Uckun FM (1999) Pokeweed antiviral protein isoforms PAP-I, PAP-II, and PAP-III depurinate RNA of human immunodeficiency virus (HIV)-1. Biochem Biophys Res Commun 260:453–458

    Article  CAS  PubMed  Google Scholar 

  • Ready MP, Brown DT, Robertus JD (1986) Extracellular localization of pokeweed protein. Proc Natl Acad Sci USA 84:5053–5056

    Google Scholar 

  • Sharma N, Park S-W, Vepachedu R, Barbieri L, Ciani M, Stirpe F, Savary BJ, Vivanco JM (2004) Isolation and characterization of an RIP-like protein from Nicotiana tabacum with dual enzymatic activity. Plant Physiol 134:171–181

    Google Scholar 

  • Valbonesi P, Barbieri L, Bolognesi A, Bonora E, Polito L, Stirpe F (1999) Preparation of highly purified momordin II without ribonuclease activity. Life Sci 65:1485–1491

    Article  CAS  PubMed  Google Scholar 

  • Vepachedu R, Bais HP, Vivanco JM (2003) Molecular characterization and post-transcriptional regulation of ME1, a type-I ribosome-inactivating protein from Mirabilis expansa. Planta 217:498–506

    Article  CAS  PubMed  Google Scholar 

  • Wang J-H, Nie H-L, Huang H, Tam S-C, Zheng Y-T (2003) Independency of anti-HIV-1 activity from ribosome-inactivating activity of trichosanthin. Biochem Biophys Res Commun 302:89–94

    Article  CAS  PubMed  Google Scholar 

  • Wang P, Tumer NE (1999) Pokeweed antiviral protein cleaves double-stranded supercoiled DNA using the same active site required to depurinate rRNA. Nucleic Acids Res 27:1900–1905

    Article  CAS  PubMed  Google Scholar 

  • Zoubenko O, Unkun F, Hur Y, Chet I, Tumer NE (1997) Plant resistance to fungal infection induced by nontoxic pokeweed antiviral protein mutants. Nat Bio/Technol 15:992–996

    Google Scholar 

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Correspondence to Jorge M. Vivanco.

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Park, SW., Vepachedu, R., Sharma, N. et al. Ribosome-inactivating proteins in plant biology. Planta 219, 1093–1096 (2004). https://doi.org/10.1007/s00425-004-1357-8

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  • DOI: https://doi.org/10.1007/s00425-004-1357-8

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