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Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex

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Abstract

We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5′-GAGTC/5′-GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I.

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Abbreviations

bp:

base pairs

dNTP:

deoxynucleoside triphosphates

DTT:

dithiothreitol

IPTG:

isopropyl-β-D-thiogalactoside

N:

nickase

ORF:

open reading frame

PCR:

polymerase chain reaction

RE, R:

restriction endonuclease

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    A. K. Yunusova, E. A. Rogulin, R. I. Artyukh & L. A. Zheleznaya

  2. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    N. I. Matvienko

Authors
  1. A. K. Yunusova
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  2. E. A. Rogulin
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  3. R. I. Artyukh
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  4. L. A. Zheleznaya
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  5. N. I. Matvienko
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Corresponding author

Correspondence to N. I. Matvienko.

Additional information

Original Russian Text © A. K. Yunusova, E. A. Rogulin, R. I. Artyukh, L. A. Zheleznaya, N. I. Matvienko, 2006, published in Biokhimiya, 2006, Vol. 71, No. 7, pp. 1002–1008.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM06-023, May 28, 2006.

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Yunusova, A.K., Rogulin, E.A., Artyukh, R.I. et al. Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex. Biochemistry (Moscow) 71, 815–820 (2006). https://doi.org/10.1134/S0006297906070157

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  • DOI: https://doi.org/10.1134/S0006297906070157

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