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Branching of the galacturonan backbone of comaruman, a pectin from the marsh cinquefoil Comarum palustre L.

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Abstract

Galacturonan, the main constituent of the backbone (core) of the comaruman macromolecule, a pectin from the marsh cinquefoil Comarum palustre L., was obtained on partial acid hydrolysis of the pectin. Using atomic force microscopy and methylation analysis of the galacturonan, the backbone of the comaruman macromolecule was shown to contain branches as side chains consisting of α-1,4-linked residues of D-galactopyranosyl uronic acid attached to the 2-and 3-positions of the galacturonic acid residues of the core, in addition to linear regions of α-1,4-D-galacturonan. A few side chains appear to attach to 2,3-positions of the D-galacturonic acid residues.

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Abbreviations

AFM:

atomic force microscopy

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Authors and Affiliations

  1. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia

    R. G. Ovodova, S. V. Popov, O. A. Bushneva, V. V. Golovchenko & Yu. S. Ovodov

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    A. O. Chizhov

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871, Moscow, Russia

    D. V. Klinov

Authors
  1. R. G. Ovodova
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  2. S. V. Popov
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  3. O. A. Bushneva
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  4. V. V. Golovchenko
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  5. A. O. Chizhov
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  6. D. V. Klinov
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  7. Yu. S. Ovodov
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Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 5, pp. 666–671.

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Ovodova, R.G., Popov, S.V., Bushneva, O.A. et al. Branching of the galacturonan backbone of comaruman, a pectin from the marsh cinquefoil Comarum palustre L.. Biochemistry (Moscow) 71, 538–542 (2006). https://doi.org/10.1134/S0006297906050117

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  • DOI: https://doi.org/10.1134/S0006297906050117

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