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Single step immobilized metal ion affinity precipitation/chromatography based procedures for purification of concanavalin A and Cajanus cajan mannose-specific lectin

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Abstract

Concanavalin A and a mannose-specific lectin could be precipitated specifically from extracts of jack bean and Cajanus cajan seeds, respectively, using metal charged EGTA. Single step purification of the lectins was also possible using iminodiacetic acid-Sepharose charged with metal ions. Nondenaturing electrophoresis in polyacrylamide gel and that performed in presence of SDS ascertained homogeneity of the isolated lectins. The migration behavior of the purified lectins was comparable with those of the lectins purified using alternative procedures.

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Abbreviations

IDA:

iminodiacetic acid

IMA:

immobilized metal ion adsorption

IMAC:

IMA chromatography

Con A:

concanavalin A

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Authors and Affiliations

  1. Interdisciplinary Biotechnology Unit and the Department of Biochemistry, Aligarh Muslim University Aligarh-202002, India

    A. Naeem, R. H. Khan & M. Saleemuddin

Authors
  1. A. Naeem
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  2. R. H. Khan
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  3. M. Saleemuddin
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Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 1, pp. 70–74.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM05-062, July 3, 2005.

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Naeem, A., Khan, R.H. & Saleemuddin, M. Single step immobilized metal ion affinity precipitation/chromatography based procedures for purification of concanavalin A and Cajanus cajan mannose-specific lectin. Biochemistry (Moscow) 71, 56–59 (2006). https://doi.org/10.1134/S0006297906010081

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  • DOI: https://doi.org/10.1134/S0006297906010081

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