Skip to main content
SpringerLink
Log in

Obtaining Chitosan from Chitine-Containing Raw Materials in Uzbekistan and Research on Antimicrobial Properties

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

The optimal conditions for the isolation of valuable natural biopolymers were revealed. Chitosan (CH) was obtained from cysts of A. parthenogenetia in the Aral Sea and from pupae of the silkworm Bombyx mori. Chitosan was obtained from cysts of A. parthenogenetia with a molecular weight of 89 kDa, a degree of deacetylation (DDA) of 72% and a solubility of 81.62%. It has been shown that it is possible to isolate CH from silkworm pupae of sufficient purity with different molecular weights and DDAs depending on the synthesis conditions and the degree of purification. Bombyx mori CH has a higher molecular weight of 100–160 kDa, a high DDA, up to 90%, and a solubility of 88%. IR spectroscopy, X-ray diffraction and elemental analysis have shown the purity of the isolated CH from various sources. In chitin from A. parthenogenetia cysts of the Aral Sea, two types of modifications, α and β, with corresponding syngonies are observed, which confirms its polymorphic structure. The antibacterial activity of B. mori CH has been shown, which tends to expand the spectrum of suppressed bacteria with increasing concentration and have a bactericidal effect on both gram-negative and gram-positive bacteria. CH of B. mori had an immunostimulating effect on living organisms, which allows us to recommend drugs based on CH to strengthen the immune system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig.1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Khitozan (Chitosan), Skryabin, K.G., Mikhailov, S.N., and Varlamov, V.P., Eds., Moscow: Tsentr Bioinzheneriya Ross. Akad. Nauk, 2013.

    Google Scholar 

  2. Caracciolo, G., Vali, H., Moore, A., and Mahmoudi, M., Nano Today, 2019, vol. 27, no. 6, p. 10. https://doi.org/10.1016/j.nantod.2019.06.001

    Article  Google Scholar 

  3. Sheik, S., Nagaraja, G.K.;., and Chandrashekar, K.R., Mater. Today Proc., 2018, vol. 5, no. 10, pp. 21011–21017.

    Article  CAS  Google Scholar 

  4. Wang, W., Meng, Q., Li, Q., Liu, J., Zhou, M., Jin, Z., and Zhao, K., Int. J. Mol. Sci., 2020, vol. 21, no. 2, p. 487. https://doi.org/10.3390/ijms21020487

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Belbekhouche, S., Bousserrhine, N., Alphonse, V., Le Floch, F., Charif Mechiche, Y., Menidjel, I., and Carbonnier, B., Colloids Surf. B, 2019, vol. 181, pp. 158–165.

    Article  CAS  Google Scholar 

  6. Le-Vinh, B., Minh, N., Le, N., Nazir, I., Matuszczak, B., and Bernkop-Schnürch, A., Int J. Biol. Macromol., 2019, vol. 133, pp. 647–655. https://doi.org/10.1016/j.ijbiomac.2019.04.081

    Article  CAS  PubMed  Google Scholar 

  7. Wang, W., Meng, Q., Li, Q., Liu, J., Zhou, M., Jin, Z., and Zhao, K., Int. J. Mol. Sci., 2020, vol. 21, p. 487. https://doi.org/10.3390/ijms21020487

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Rashidova, S.Sh. and Milusheva, R.Yu., Khitin i khitozan Bombyx mori. Sintez, svoistva i primenenie (Chitin and Chitosan from Bombyx mori: Synthesis, Properties, and Applications), Tashkent: FAN, 2009.

  9. Milusheva, R.Yu., Avazova, O.B., and Rashidova, S.Sh., Belok iz kukolok tutovogo shelkopryada Bombyx mori L. Vydelenie, svoistva, primenenie (Protein from Silkworm Bombyx mori L. Pupae: Extraction, Properties, and Application). Tashkent: FAN, 2020.

  10. Mirabdullayev, I., Abdullaeva, L., Musaev, A., Zholdasova, I., Mustafaeva, Z., and Jumaniezova, N., Geophys. Res. Abstr., 2007, vol. 9, p. 772.

    Google Scholar 

  11. Aladin, N.V. and Plotnikov, I.S., Proc. Zool. Inst. Ross. Akad. Nauk, 2008, vol. 312, nos. 1–2, pp. 145–154.

    Article  Google Scholar 

  12. Rudneva, I.I., Artemia/Prospects for Use in the National Economy, Kiev: Naukova Dumka, 1991.

    Google Scholar 

  13. Ravikumar, N.V., React. Funct. Polym., 2000, vol. 46, pp. 1–27.

    Article  Google Scholar 

  14. Muzzarelli, R.A.A., The discovery of chitin, in Chitosan in Pharmacy and Chemistry, Muzzarelli, R.A.A. and Muzzarelli, C., Eds., Italy: Atec., 2002, pp. 1–8.

    Google Scholar 

  15. Milusheva, R.Yu., Pirniyazov, K.K., and Rashidova, S.Sh., Vestn. Tver. Gos. Univ., Ser.: Khim., 2016, no. 2, pp. 119–124.

  16. Gamzazade, A.I., Shlimak, B.M., Sklyar, A.M., Stykova, E.V., Pavlova, S.A., and Rogozin, S.V., Acta Polym., 1985, vol. 36, no. 8, pp. 421–424.

    Article  Google Scholar 

  17. Pogodina, N.V., Pavlov, G.M., Bunin, S.V., and Men’shikov, A.V., Vysokomol. Soed., 1986, no. 28, pp. 232–239.

  18. Gosudarstvennaya farmakopeya SSSR. XI (State Pharmacopoeia of the USSR. XI), Moscow: Meditsina, 2012, vol. 2, p. 24.

  19. Gosudarstvennaya farmakopeya SSSR XI (State Pharmacopoeia of the USSR. XI), Moscow: Meditsina, 1987, vol. 1, p. 175.

  20. Klimova V.A. Osnovnye mikrometody analiza organicheskikh soedinenii. Moskva: Khimiya, 2001. 75 s.

  21. Litvinenko, L.I. and Boiko, E.G., Biol. Vnutr. Vod, 2008, no. 1, pp. 40–48.

    Google Scholar 

  22. Bansal, V., Sharma, P.K., and Sharma, N., Adv. Biol. Res., 2011, vol. 5, no. 1, pp. 28–37.

    CAS  Google Scholar 

  23. Rinaudo, M., Prog. Polym. Sci., 2006, vol. 31, pp. 603–632.

    Article  CAS  Google Scholar 

  24. Kumar, M.R., Muzzarelli, R.A.A., Muzzarelli, C., Sashiva, H., and Domb, A.J., Chem. Rev., 2004, vol. 104, pp. 6017–6084.

    Article  PubMed  Google Scholar 

  25. Yugai, S.M., Shakhobutdinov, S.Sh., Atakhanov, A.A., and Rashidova, S.Sh, Khim. Zh. Kaz., 2019, vol. 67, no. 3, pp. 254–265.

    Google Scholar 

  26. Khazhibaev, K.G. Berdimbetova, G.E., Karlybaeva, B.P., and Oshchepkova, Yu.I., Universum: Khim. Biol., 2019, no. 9, p. 63. http://7universum.com/ru/nature/archive/item/7769.

  27. Il'ina, A.V., Varlamov, V.P., and Ermakov, Yu.A., Dokl. Akad. Nauk, 2008, vol. 421, no. 2, pp. 199–201.

    Google Scholar 

  28. Chen, Y.M., Chung, Y.C., Wang, L.W., Chen, K.T., and Li, S.Y., J. Environ. Sci. Health, Part A: Toxic/Hazard. Subst. Environ. Eng., 2002, vol. 37, p. 1379.

    Article  Google Scholar 

Download references

Funding

This work was financed from the budget of the institute (Institute of Chemistry and Physics of Polymers of the Academy of Sciences of the Republic of Uzbekistan, Uzbekistan). No additional grants were received to conduct or direct this specific study.

Author information

Authors and Affiliations

  1. Institute of Polymer Chemistry and Physics, Academy of Sciences of the Republic of Uzbekistan, 100128, Tashkent, Uzbekistan

    R. Yu. Milusheva, G. D. Akhymbetova & S. Sh. Rashidova

Authors
  1. R. Yu. Milusheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. D. Akhymbetova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Sh. Rashidova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Yu. Milusheva.

Ethics declarations

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

The maintenance and handling of laboratory animals was carried out in accordance with the requirements of the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes and the legislation and adopted standards of the Academy of Sciences of the Republic of Uzbekistan.

CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

Additional information

The article has been presented at the XVI All-Russian Conference with International Participation “Modern Perspectives in Chitin and Chitosan Studies” (RosChit-2023, https://roschit23.ru).

Publisher’s Note.

Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Milusheva, R.Y., Akhymbetova, G.D. & Rashidova, S.S. Obtaining Chitosan from Chitine-Containing Raw Materials in Uzbekistan and Research on Antimicrobial Properties. Appl Biochem Microbiol 60, 216–225 (2024). https://doi.org/10.1134/S0003683824020108

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683824020108

Keywords:

Search

Navigation