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Effect of Bacillus subtilis and Signaling Molecules on the State of the Pro/Antioxidant System and the Expression of Protective Protein Genes in Potato Plants upon Phytophthorosis and a Moisture Deficit

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Abstract

The effect of the bacteria Bacillus subtilis in combination with salicylic (SA) and jasmonic (JA) acids on the state of the pro/antioxidant system (the content of hydrogen peroxide, the activity of catalase, peroxidase, superoxide dismutase), the transcriptional activity of the genes PR-1, PR-6, and PR-9, and changes in the leaf proteome in connection with the development of potato resistance to the causative agent of late blight, the oomycete Phytophthora infestans (Mont.) de Bary, upon a moisture deficiency was studied. Plants grown from microtubers were sprayed with a suspension of B. subtilis (108 cells/mL) and a mixture of bacteria with SA (10–6 M), JA (10–7 M), SA + JA. Three days after treatment, the plants were infected with P. infestans (105 spores/mL) and cultivated under artificial soil drought conditions created with a reduction in irrigation. The biochemical parameters in plants were assessed when the soil moisture reached 40 ± 5% of full moisture capacity (7 days after infection). A decrease in the degree of P. infestans development on leaves was detected upon treatment with B. subtilis in combination with SA and JA. The mechanism of the activation of the defense systems of potato plants by bacteria of the genus Bacillus and signaling molecules under drought conditions was associated with the accumulation of H2O2, an increase in the activity of antioxidant enzymes, and the expression of PR-protein genes. In the proteome of potato leaves, differences were found in the presence of 19 polypeptides in the pI range from 4.0 to 9.0 with molecular weights from 30 to 125 kDa. Treatment with B. subtilis in combination with JA most significantly changed the protein spectra in both healthy and infected plants. The revealed differences in the activation of the synthesis of protective proteins under the influence of B. subtilis bacteria and signaling molecules indicate the existence of differential pathways for the formation of resistance to P. infestans in potato plants with their participation.

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ACKNOWLEDGMENTS

The work was carried out on the equipment of Biomika Core Facility Center (Department of Biochemical Research and Nanobiotechnology of the Agidel Regional Center for Collective Use) and the Unique Scientific Installation Kodink.

Funding

The work was partially funded within the framework of government task (state registration number AAAA-A21-121011990120-7), by the Russian Foundation for Basic Research, and by the Belarusian Republican Foundation for Basic Research (scientific project no. 20-516-00005).

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, separate structural unit of the Ufa Federal Research Center, Russian Academy of Sciences, 450054, Ufa, Russia

    L. G. Yarullina, G. F. Burkhanova, E. A. Cherepanova, A. V. Sorokan & E. A. Zaikina

  2. Bashkir State University, 450076, Ufa, Russia

    V. O. Tsvetkov

  3. Bashkir Research Institute of Agriculture, a separate structural unit of the Ufa Federal Research Center, Russian Academy of Sciences, 450059, Ufa, Russia

    I. S. Mardanshin

  4. Kuprevich Institute of Experimental Botany, National Academy of Sciences of Belarus, 220072, Minsk, Belarus

    J. N. Kalatskaya & N. V. Balyuk

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  1. L. G. Yarullina
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  2. V. O. Tsvetkov
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  3. G. F. Burkhanova
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  6. E. A. Zaikina
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  8. J. N. Kalatskaya
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Correspondence to L. G. Yarullina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Bulaev

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Yarullina, L.G., Tsvetkov, V.O., Burkhanova, G.F. et al. Effect of Bacillus subtilis and Signaling Molecules on the State of the Pro/Antioxidant System and the Expression of Protective Protein Genes in Potato Plants upon Phytophthorosis and a Moisture Deficit. Appl Biochem Microbiol 57, 760–769 (2021). https://doi.org/10.1134/S0003683821060132

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